Quadruplet codons allow multiplexing of non-canonical amino acids within single polypeptides in living cells. We show that including high-usage triplet codons after quadruplet codons can improve their decoding efficiency in genetic circuits, which allowed us to develop a system for the programmable biosynthesis of exotic macrocyclic peptides in cells.

中文翻译：

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用于多重遗传密码扩展的重新编码基因电路


四胞胎密码子允许在活细胞的单个多肽内对非经典氨基酸进行多重检测。我们表明，在四胞胎密码子之后包含高使用率的三联体密码子可以提高它们在遗传电路中的解码效率，这使我们能够开发一种用于细胞中外来大环肽的可编程生物合成的系统。