Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Microfluidic sperm trap array for single-cell flagellar analysis with unrestricted 2D flagellar movement
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-09-12 , DOI: 10.1039/d4lc00515e Kaiyu Wang 1 , Antai Tao 1 , Rongjing Zhang 1 , Junhua Yuan 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-09-12 , DOI: 10.1039/d4lc00515e Kaiyu Wang 1 , Antai Tao 1 , Rongjing Zhang 1 , Junhua Yuan 1
Affiliation
|
Sperm capture techniques that immobilize sperm to halt their motility are essential for the long-term analysis of individual sperm. These techniques are beneficial in assisted reproductive technologies such as intracytoplasmic sperm injection (ICSI) by allowing selective screening of sperm. However, there is a notable lack of high-throughput and non-destructive sperm capture methods that allow the flagellum to beat freely, which is crucial for accurately reflecting the behavior of unfettered, freely swimming sperm. To bridge this gap, we introduce a novel microfluidic device specifically engineered to capture sperm without restricting flagellar motion. The design utilizes sperm's innate boundary-following behavior in both 3D and 2D environments to direct them into a capture zone. Once captured, the sperm head is restrained while the flagellum remains free to exhibit natural beating patterns. Utilizing this device, we explore the effects of hyperactivating agents, temperature, and their combined influence on the dynamics of bovine sperm flagella. The unrestricted flagellar motion offered by our device yields two prominent advantages: it mirrors the flagellar behavior of free-swimming sperm, ensuring research findings are consistent with natural sperm activity, and it prevents imaging overlap between the flagellum and the capture structures, simplifying the automation of flagellar tracking and analysis. This technological advancement facilitates the collection of waveform parameters along the entire flagellum, addressing inconsistencies that have arisen in previous research due to differing measurement sites, and enabling precise extraction of sperm behavioral properties.
中文翻译：
微流控精子捕获芯片，用于单细胞鞭毛分析，具有不受限制的 2D 鞭毛运动
固定精子以阻止其运动的精子捕获技术对于单个精子的长期分析至关重要。这些技术在辅助生殖技术中是有益的，例如卵胞浆内单精子注射 （ICSI），允许对精子进行选择性筛选。然而，明显缺乏允许鞭毛自由跳动的高通量和非破坏性精子捕获方法，这对于准确反映不受约束、自由游泳的精子的行为至关重要。为了弥合这一差距，我们推出了一种新型微流控装置，专门设计用于在不限制鞭毛运动的情况下捕获精子。该设计利用精子在 3D 和 2D 环境中固有的边界跟随行为将其引导到捕获区。一旦被捕获，精子头就会被束缚，而鞭毛仍然自由地表现出自然的跳动模式。利用该设备，我们探讨了过度激活剂、温度及其对牛精子鞭毛动力学的综合影响。我们的设备提供的不受限制的鞭毛运动产生了两个突出的优势：它反映了自由游泳精子的鞭毛行为，确保研究结果与自然精子活动一致，并且它防止了鞭毛和捕获结构之间的成像重叠，简化了鞭毛跟踪和分析的自动化。这项技术进步有助于沿整个鞭毛收集波形参数，解决了以前研究中由于测量位点不同而出现的不一致问题，并能够精确提取精子的行为特性。
更新日期：2024-09-12
中文翻译：
微流控精子捕获芯片，用于单细胞鞭毛分析，具有不受限制的 2D 鞭毛运动
固定精子以阻止其运动的精子捕获技术对于单个精子的长期分析至关重要。这些技术在辅助生殖技术中是有益的，例如卵胞浆内单精子注射 （ICSI），允许对精子进行选择性筛选。然而，明显缺乏允许鞭毛自由跳动的高通量和非破坏性精子捕获方法，这对于准确反映不受约束、自由游泳的精子的行为至关重要。为了弥合这一差距，我们推出了一种新型微流控装置，专门设计用于在不限制鞭毛运动的情况下捕获精子。该设计利用精子在 3D 和 2D 环境中固有的边界跟随行为将其引导到捕获区。一旦被捕获，精子头就会被束缚，而鞭毛仍然自由地表现出自然的跳动模式。利用该设备，我们探讨了过度激活剂、温度及其对牛精子鞭毛动力学的综合影响。我们的设备提供的不受限制的鞭毛运动产生了两个突出的优势：它反映了自由游泳精子的鞭毛行为，确保研究结果与自然精子活动一致，并且它防止了鞭毛和捕获结构之间的成像重叠，简化了鞭毛跟踪和分析的自动化。这项技术进步有助于沿整个鞭毛收集波形参数，解决了以前研究中由于测量位点不同而出现的不一致问题，并能够精确提取精子的行为特性。
京公网安备 11010802027423号