Attraction of Nicotiana benthamiana to Bemisia tabaci is related to a chemical signal in plant volatile, undecane,Pest Management Science

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Attraction of Nicotiana benthamiana to Bemisia tabaci is related to a chemical signal in plant volatile, undecane
Pest Management Science ( IF 3.8 ) Pub Date : 2024-09-11 , DOI: 10.1002/ps.8411
Xin-Yue Zhang ₁ , Wen-Hao Han ₁ , Feng-Bin Zhang ₁ , Jun-Xia Wang ₁ , Shu-Sheng Liu ₁ , Xiao-Wei Wang ₁

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BACKGROUNDThe whitefly Bemisia tabaci is one of the world's foremost agricultural pests. Recently, we found that a wild relative of tobacco (Nicotiana benthamiana) demonstrates remarkable attractiveness and nearly 100% lethality towards whiteflies. Therefore, it can act as a dead‐end trap crop for whitefly control in the field. However, the underlying mechanism of the significant attractiveness of N. benthamiana towards whiteflies is unclear.RESULTSBinary‐choice assays and olfactory experiments showed that compared to common tobacco (N. tabacum), the volatile of N. benthamiana has a greater attraction to whiteflies. Then we analyzed and compared volatiles from these two Nicotiana species by thermal desorption‐gas chromatography–mass spectrometry (TD‐GC–MS). We identified 16 chemical compounds that are more abundant in N. benthamiana than in N. tabacum. Seven compounds were further tested with olfactometer assays and we found that, among them, undecane strongly attracted whiteflies. Further experiments revealed that even 0.005 μg mL−1 undecane is attractive to whiteflies. We also silenced the genes that may influence the biosynthesis of undecane and found the production of undecane decreased after silencing NbCER3, and that N. benthamiana plants with less undecane lost their attraction to whiteflies. In addition, we found that applying 0.005 μg mL−1 undecane on yellow sticky traps can increase the number of stuck insects on the traps by ≈40%.CONCLUSIONUndecane from the volatile of N. benthamiana is a critical chemical signal that attracts whiteflies and NbCER3 involved in the biosynthesis of undecane. Undecane may be used to improve the efficiency of yellow sticky traps for whitefly control. © 2024 Society of Chemical Industry.

中文翻译：

本塞姆氏烟草对烟粉虱的吸引力与植物挥发性十一烷中的化学信号有关

背景技术烟粉虱(Bemisia tabaci)是世界上最重要的农业害虫之一。最近，我们发现烟草的一种野生近缘种（Nicotiana Benthamiana）对粉虱具有显着的吸引力和近 100% 的致死率。因此，它可以作为田间粉虱防治的死胡同诱捕作物。然而，本塞姆氏烟草对粉虱具有显着吸引力的潜在机制尚不清楚。结果二元选择分析和嗅觉实验表明，与普通烟草（N. tabacum）相比，本氏烟草的挥发性成分对粉虱具有更大的吸引力。然后，我们通过热解吸气相色谱-质谱法（TD-GC-MS）分析和比较了这两种烟草的挥发物。我们鉴定出 16 种化合物在本塞姆氏烟草中比在烟草中更丰富。通过嗅觉计检测进一步测试了七种化合物，我们发现其中十一烷对粉虱具有强烈的吸引力。进一步的实验表明，即使是 0.005 μg mL−1 十一烷也能吸引粉虱。我们还沉默了可能影响十一烷生物合成的基因，发现沉默NbCER3后十一烷的产量减少，并且十一烷较少的本塞姆氏烟草植物失去了对粉虱的吸引力。此外，我们发现在黄色粘虫诱捕器上施用 0.005 μg mL−1 十一烷可以使诱捕器上被困昆虫的数量增加约 40%。结论本塞姆氏烟草挥发物中的十一烷是吸引粉虱和 NbCER3 的关键化学信号参与十一烷的生物合成。十一烷可用于提高黄色粘虫捕捉粉虱的效率。 © 2024 化学工业协会。

更新日期：2024-09-11

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