UV/Visible Diffusion-Ordered Spectroscopy: A Simultaneous Probe of Molecular Size and Electronic Absorption,Analytical Chemistry

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UV/Visible Diffusion-Ordered Spectroscopy: A Simultaneous Probe of Molecular Size and Electronic Absorption
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-10 , DOI: 10.1021/acs.analchem.4c02026
Giulia Giubertoni ₁ , Marina Gomes Rachid ₁ , Carolyn Moll ₁ , Michiel Hilbers ₁ , Saer Samanipour ₁ , Sander Woutersen ₁

Affiliation

Based on concepts from nuclear magnetic resonance, we have developed UV/vis diffusion-ordered spectroscopy, which simultaneously probes the size and electronic absorption spectrum of molecules and particles. We use simple flow technology to create a step-function concentration profile inside an optical sample cell, and by measuring the time-dependent absorption spectrum in an initially solvent-filled part of the sample volume, we obtain the diffusion coefficients and UV/vis spectra of the species present in the sample solution. From these data, we construct a two-dimensional spectrum with absorption wavelength on one axis and diffusion coefficient (or equivalently, size) on the other, in which the UV/vis spectrum of a mixture with different molecular sizes is separated into the spectra of the different species, sorted by size. We demonstrate this method on mixed solutions of fluorescent dyes, biomolecules, and the UV-absorbing components of coffee, caffeine, and chlorogenic acid, all with concentrations in the μM range.

中文翻译：

紫外/可见扩散有序光谱：分子大小和电子吸收的同步探针

基于核磁共振的概念，我们开发了紫外/可见扩散有序光谱，可同时探测分子和颗粒的尺寸和电子吸收光谱。我们使用简单的流动技术在光学样品池内创建阶跃函数浓度分布，并通过测量样品体积中最初充满溶剂的部分中的时间相关吸收光谱，我们获得扩散系数和紫外/可见光谱样品溶液中存在的物种。根据这些数据，我们构建了一个二维光谱，其一轴为吸收波长，另一轴为扩散系数（或等效的尺寸），其中具有不同分子尺寸的混合物的紫外/可见光谱被分成以下光谱：不同的物种，按大小排序。我们在荧光染料、生物分子以及咖啡、咖啡因和绿原酸的紫外线吸收成分的混合溶液中演示了这种方法，所有浓度均在 μM 范围内。

更新日期：2024-09-10

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