Citrus Fruits Produce Direct Defense Responses against Oviposition by Bactrocera minax (Diptera: Tephritidae),Journal of Agricultural and Food Chemistry

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Citrus Fruits Produce Direct Defense Responses against Oviposition by Bactrocera minax (Diptera: Tephritidae)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-11 , DOI: 10.1021/acs.jafc.4c05871
Bingbing Wei ₁ , Shuai Cao ₁ , Guijian Zhang ₁ , Haoran Wang ₁ , Zhen Cao ₁ , Qiaoran Chen ₁ , Changying Niu ₁

Affiliation

Plants perceive and orchestrate defense responses when herbivorous insects are ovipositing. Fruits, as a crucial reproductive organ in plants, have rarely been researched on the responses to insect eggs. Here, we found that oviposition by the specialist insect Bactrocera minax in navel oranges activated the lignin synthesis pathway and cell division, causing mechanical pressure that crushed the eggs. Transcriptome and metabolome analyses revealed an enrichment of oviposition-induced genes and metabolites within the lignin synthesis pathway, which was confirmed by histochemical staining. Furthermore, hydrogen peroxide (H₂O₂) accumulation was observed at the oviposition sites. Plant defense-related hormones jasmonic acid (JA) and salicylic acid (SA) exhibited rapid induction after oviposition, while indole-3-acetic acid (IAA) activation occurred in the later stages of oviposition. Additionally, secondary metabolites induced by prior egg deposition were found to influence larval performance. Our studies provide molecular evidence that host fruits have evolved defense mechanisms against insect eggs and pave the way for future development of insect-resistant citrus varieties.

中文翻译：

柑橘类水果对 Bactrocera minax （Diptera： Tephritidae）的产卵产生直接防御反应

当食草昆虫产卵时，植物会感知并协调防御反应。水果作为植物重要的生殖器官，很少研究对虫卵的反应。在这里，我们发现专业昆虫 Bactrocera minax 在脐橙中的产卵激活了木质素合成途径和细胞分裂，从而产生压碎卵的机械压力。转录组和代谢组分析显示木质素合成途径中产卵诱导的基因和代谢物富集，这可以通过组织化学染色得到证实。此外，在产卵部位观察到过氧化氢（H₂O₂）积累。植物防御相关激素茉莉酸（JA）和水杨酸（SA）在产卵后表现出快速诱导，而吲哚-3-乙酸（IAA）激活发生在产卵后期。此外，发现先前卵沉积诱导的次生代谢物会影响幼虫的性能。我们的研究提供了分子证据，表明寄主果实已经进化出对虫卵的防御机制，并为抗虫柑橘品种的未来发展铺平了道路。

更新日期：2024-09-11

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