The simultaneous enrichment of the secoiridoid glycosides gentiopicroside, loganic acid, swertiamaroside, and sweroside from amino acid-based deep eutectic solvent (AADES) extracts of roots and rhizomes was developed. To screen suitable resins, the performance and characterization of 11 widely used macroporous resins for the adsorption and desorption of target secoiridoid glycosides were considered. Compared with the other resins, the H103 resin was found to have greater adsorption and desorption capacities for gentiopicroside, loganic acid, swertiamaroside, and sweroside. Therefore, this resin was chosen as the best resin for secoiridoid glycosides enrichment. In a column packed with H103 resin, the dynamic adsorption and desorption factors for the target secoiridoid glycosides were investigated and optimized. After dynamic testing on the H103 packed column, the optimal operating conditions were determined as follows: a sample volume of 12 bed volumes (BVs) and a loading flow rate of 2 BV/h were used, the volume of deionized water used for the aqueous washing process was 8 BV, and the aqueous washing flow rate was 3 BV/h. Ethanol with a volume fraction of 10–90 % was used as the eluent in the ethanol desorption process with a gradient of 10 %. Each ethanol volume fraction was used for 2 BV, with a flow rate of 3 BV/h. The eluent fractions containing 40–80 % ethanol were collected, combined, and concentrated, followed by vacuum drying to obtain the final product. The purities of gentiopicroside, loganic acid, swertiamaroside, and sweroside in the final product were 58.11, 13.73, 3.86, and 2.77 %, respectively, with corresponding recovery rates of 74.09, 72.19, 77.50, and 76.45 %, respectively. Additionally, the reuse of AADES proved to be effective.

中文翻译：

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大孔树脂吸附在氨基酸低共熔溶剂中富集六龙胆提取物中的环烯醚萜苷


开发了从根和根茎的基于氨基酸的低共熔溶剂 (AADES) 提取物中同时富集环烯醚萜苷类龙胆苦苷、马钱酸、獐牙菜苷和獐牙菜苷的方法。为了筛选合适的树脂，考虑了 11 种广泛使用的大孔树脂用于目标环烯醚萜苷的吸附和解吸的性能和表征。与其他树脂相比，H103 树脂对龙胆苦苷、马钱酸、獐牙菜苷和獐牙菜苷具有更大的吸附和解吸能力。因此，选择该树脂作为富集环烯醚萜苷的最佳树脂。在填充 H103 树脂的色谱柱中，研究并优化了目标环烯醚萜苷的动态吸附和解吸因子。经过对H103填充柱的动态测试，确定最佳操作条件为：样品体积为12床体积（BV），上样流速为2 BV/h，水相用去离子水体积洗涤过程为8 BV，水洗流速为3 BV/h。乙醇解吸过程中使用体积分数为10%~90%的乙醇作为洗脱液，梯度为10%。每个乙醇体积分数用于2 BV，流速为3 BV/h。收集含40%~80%乙醇的洗脱液，合并、浓缩，真空干燥即得最终产品。最终产品中龙胆苦苷、马钱酸、獐牙菜苷和獐牙菜苷的纯度分别为58.11、13.73、3.86和2.77%，相应的回收率分别为74.09、72.19、77.50和76.45%。此外，AADES 的重复使用被证明是有效的。