Nature Communications ( IF 14.7 ) Pub Date : 2024-09-10 , DOI: 10.1038/s41467-024-52339-w
Yasuhito Sakuraba 1 , Mailun Yang 1 , Shuichi Yanagisawa 1
Nitrogen (N) deficiency responses are essential for plant survival and reproduction. Here, via an expression genome-wide association study (eGWAS), we reveal a mechanism that regulates microRNA (miRNA) dynamics necessary for N deficiency responses in Arabidopsis. Differential expression levels of three NAC transcription factor (TF) genes involved in leaf N deficiency responses among Arabidopsis accessions are most significantly associated with polymorphisms in HASTY (HST), which encodes an importin/exportin family protein responsible for the generation of mature miRNAs. HST acts as a negative regulator of N deficiency-induced leaf senescence, and the disruption and overexpression of HST differently modifies miRNA dynamics in response to N deficiency, altering levels of miRNAs targeting transcripts. Interestingly, N deficiency prevents the interaction of HST with HST-interacting proteins, DCL1 and RAN1, and some miRNAs. This suggests that HST-mediated regulation of miRNA dynamics collectively controls regulations mediated by multiple N deficiency response-associated NAC TFs, thereby being central to the N deficiency response network.
中文翻译:
HASTY 介导的 miRNA 动力学调节拟南芥氮饥饿诱导的叶片衰老
氮 (N) 缺乏反应对于植物的生存和繁殖至关重要。在这里,通过表达全基因组关联研究 (eGWAS),我们揭示了调节拟南芥中 N 缺乏反应所必需的 microRNA (miRNA) 动力学的机制。拟南芥种质中参与叶氮缺乏反应的 3 个 NAC 转录因子 (TF) 基因的差异表达水平与 HASTY (HST) 多态性最显著相关,HST 编码负责产生成熟 miRNA 的输入素/输出素家族蛋白。HST 作为 N 缺乏诱导的叶片衰老的负调节因子,HST 的破坏和过表达以不同的方式改变响应 N 缺乏的 miRNA 动力学,从而改变靶向转录本的 miRNA 水平。有趣的是,氮缺乏会阻止 HST 与 HST 相互作用蛋白 DCL1 和 RAN1 以及一些 miRNA 的相互作用。这表明 HST 介导的 miRNA 动力学调节共同控制由多个 N 缺陷反应相关的 NAC TFs 介导的调节,因此是 N 缺陷反应网络的核心。