STAT5B, Akt and p38 Signaling Activate FTZ-F1 to Regulate the Xenobiotic Tolerance-Related Gene SlCyp9a75b in Spodoptera litura,Journal of Agricultural and Food Chemistry

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STAT5B, Akt and p38 Signaling Activate FTZ-F1 to Regulate the Xenobiotic Tolerance-Related Gene SlCyp9a75b in Spodoptera litura
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-10 , DOI: 10.1021/acs.jafc.4c04465
Jianyi Li ₁ , Long Jin ₁ , Kunpeng Yan ₁ , Pengjun Xu ₂ , Yiou Pan ₁ , Qingli Shang ₁

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Cytochrome P450 monooxygenases in insects have been verified to implicated in insecticide and phytochemical detoxification metabolism. However, the regulation of P450s, which are modulated by signal-regulated transcription factors (TFs), is less well studied in insects. Here, we found that the Malpighian tubule specific P450 gene SlCYP9A75b in Spodoptera litura is induced by xenobiotics. The transgenic Drosophila bioassay and RNAi results indicated that this P450 gene contributes to α-cypermethrin, cyantraniliprole, and nicotine tolerance. In addition, functional analysis revealed that the MAPKs p38, PI3K/Akt, and JAK-STAT activate the transcription factor fushi tarazu factor 1 (FTZ-F1) to regulate CYP9A75b expression. These findings provide mechanistic insights into the contributions of CYP9A genes to xenobiotic detoxification and support the possible involvement of different signaling pathways and TFs in tolerance to xenobiotics in insects.

中文翻译：

STAT5B、Akt 和 p38 信号传导激活 FTZ-F1 调节斜纹夜蛾中异生素耐受性相关基因 SlCyp9a75b

昆虫中的细胞色素 P450 单加氧酶已被证实与杀虫剂和植物化学解毒代谢有关。然而，由信号调节转录因子 (TF) 调节的 P450 的调节在昆虫中的研究较少。在这里，我们发现斜纹夜蛾中的马氏小管特异性 P450 基因SlCYP9A75b是由异生素诱导的。转基因果蝇生物测定和 RNAi 结果表明，该 P450 基因有助于 α-氯氰菊酯、氰虫酰胺和尼古丁耐受。此外，功能分析表明，MAPK p38、PI3K/Akt 和 JAK-STAT 激活转录因子 fushi tarazu 因子 1 ( FTZ-F1 ) 来调节CYP9A75b表达。这些发现为 CYP9A 基因对异生物质解毒的贡献提供了机制见解，并支持不同信号通路和 TF 可能参与昆虫对异生物质的耐受性。

更新日期：2024-09-10

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