Antifungal Activity and Mechanism of Diaporthein B against Botryosphaeria dothidea in Prevention of Apple Ring Rot,Journal of Agricultural and Food Chemistry

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Antifungal Activity and Mechanism of Diaporthein B against Botryosphaeria dothidea in Prevention of Apple Ring Rot
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-10 , DOI: 10.1021/acs.jafc.4c06101
Hui-Fang Du ₁ , Ya-Hui Zhang ₁ , Wan Li ₁ , Huajie Zhu ₂ , Sen Pang ₃ , Da-Bin Song ₁ , Zhongcheng Liu ₁ , Charles U Pittman ₄ , Fei Cao ₁

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Apple ring rot, caused by the pathogenic fungus Botryosphaeria dothidea, has inflicted substantial economic losses and caused significant food safety concerns. In this study, a pimarane-type diterpenoid, diaporthein B (DTB), isolated from a marine-derived fungus, exhibited significant antifungal activity against B. dothidea, with an EC₅₀ value of 8.8 μg/mL. Transcriptome, metabolome, and physiological assays revealed that DTB may target mitochondria and disrupt the tricarboxylic acid (TCA) cycle and oxidative phosphorylation processes. This interference led to increased accumulation of reactive oxygen species and subsequent lipid peroxidation, ultimately inhibiting fungal growth. Furthermore, DTB exhibited an inhibitory potency against apple ring rot at a concentration of 31.2 μg/mL, achieving rates ranging from 67.7 to 81.6% across four distinct apple cultivars. These results indicated that DTB could serve as a novel fungicide for controlling apple ring rot in apple cultivation, transportation, and storage.

中文翻译：

甘草酸 B 防治苹果环腐病的抗真菌活性及机制

苹果环腐病由病原真菌Botryosphaeria dothidea引起，造成了巨大的经济损失，并引发了重大的食品安全问题。在这项研究中，从海洋源性真菌中分离出的一种螺环二萜类二萜化合物 diaporthein B (DTB)，对B. dothidea表现出显着的抗真菌活性，EC ₅₀值为 8.8 μg/mL。转录组、代谢组和生理学测定表明，DTB 可能靶向线粒体并破坏三羧酸 (TCA) 循环和氧化磷酸化过程。这种干扰导致活性氧的积累增加以及随后的脂质过氧化，最终抑制真菌生长。此外，DTB 在浓度为 31.2 μg/mL 时表现出对苹果环腐病的抑制效力，在四个不同的苹果品种中实现了 67.7% 至 81.6% 的抑制率。这些结果表明，DTB可以作为一种新型杀菌剂，用于防治苹果种植、运输和储存过程中的苹果环腐病。

更新日期：2024-09-10

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