Artificial turf, a consumer product growing in usage in the United States, contains diverse chemicals, some of which are endocrine disruptive. Endocrine effects from turf material extracts have been primarily limited to one component, crumb rubber, of these multi-material products. We present in vitro bioactivities from non-weathered and weathered turf sample extracts, including multiple turf components. All weathered samples were collected from real-world turf fields. Non-weathered versus weathered differentially affected the androgen (AR), estrogen (ER), glucocorticoid (GR), and thyroid receptors (TR) in reporter bioassays. While weathered extracts more efficaciously activated peroxisome proliferator activated receptor γ (PPARγ), this did not translate to greater in vitro adipogenic potential. All turf extracts activated the aryl hydrocarbon receptor (AhR). High AhR-efficacy extracts induced modest rat cardiomyoblast toxicity in an AhR-dependent manner. Our data demonstrate potential endocrine and cardiometabolic effects from artificial turf material extracts, warranting further investigation into potential exposures and human health effects.

中文翻译：

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与人造草坪材料相关的体外内分泌和心脏代谢毒性


人造草坪是一种在美国使用越来越多的消费品，它含有多种化学物质，其中一些会干扰内分泌。草坪材料提取物的内分泌影响主要局限于这些多材料产品的一种成分，即碎橡胶。我们介绍了来自非风化和风化草坪样品提取物的体外生物活性，包括多种草坪成分。所有风化样品均从真实世界的草坪场地收集。在报告基因生物测定中，非风化与风化对雄激素 （AR） 、雌激素 （ER） 、糖皮质激素 （GR） 和甲状腺受体 （TR） 的影响不同。虽然风化提取物更有效地激活了过氧化物酶体增殖物激活受体γ （PPARγ），但这并没有转化为更大的体外成脂潜力。所有草皮提取物都激活了芳烃受体 （AhR）。高 AhR 功效提取物以 AhR 依赖性方式诱导适度大鼠成心肌细胞毒性。我们的数据表明，人造草坪材料提取物对内分泌和心脏代谢有潜在影响，因此有必要进一步研究潜在的暴露和人类健康影响。