Copper Single-Atom Nanozyme Mimicking Galactose Oxidase with Superior Catalytic Activity and Selectivity,Small

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Copper Single-Atom Nanozyme Mimicking Galactose Oxidase with Superior Catalytic Activity and Selectivity
Small ( IF 13.0 ) Pub Date : 2024-09-09 , DOI: 10.1002/smll.202405986
Eslam M Hamed _{1,

2} , Limo He ₁ , Varun Rai ₃ , Song Hu ₄ , Sam F Y Li ₁

Affiliation

Due to the low stability and high cost of some natural enzymes, nanozymes have been developed as enzyme-imitating nanomaterials. Single-atom nanozymes are a class of nanozymes with metal centers that mimic the structure of metal-based natural enzymes. Herein, Cu-N-C single-atom nanozyme (SAN) is synthesized with excellent peroxidase- and enhanced oxidase-like activities to mimic the action of natural galactose oxidase. Cu-SAN demonstrates stereospecific activity akin to that of natural galactose oxidase by oxidizing D-galactose and primary alcohol but not L-Galactose or other carbohydrates. The SAN can catalyze the oxidation of galactose in the presence of oxygen, producing hydrogen peroxide as a sub-product. The produced hydrogen peroxide then oxidizes 3,3′,5,5′-tetramethylbenzidine catalyzed by the SAN, yielding the typical blue product. The relationship between absorbance and galactose concentration is linear in the 1–60 µm range with a detection limit as low as 0.23 µm. This strategy can be utilized in the diagnosis of galactosemia disorder and detection of galactose in some dairy and other commercial products. DFT calculations clarify the high activity of the Cu sites in the POD-like reaction and explain the selectivity of the Cu-SAN oxidase-like reaction toward D-galactose

中文翻译：

模拟半乳糖氧化酶的铜单原子纳米酶，具有优异的催化活性和选择性

由于一些天然酶的稳定性低、成本高，纳米酶已被开发为模拟酶的纳米材料。单原子纳米酶是一类具有金属中心的纳米酶，可模拟金属基天然酶的结构。在此，Cu-N-C 单原子纳米酶（SAN）合成了具有优异的过氧化物酶和增强的氧化酶样活性，以模拟天然半乳糖氧化酶的作用。Cu-SAN 通过氧化 D-半乳糖和伯醇而不是 L-半乳糖或其他碳水化合物来表现出类似于天然半乳糖氧化酶的立体特异性活性。SAN 可以在氧气存在下催化半乳糖的氧化，产生过氧化氢作为副产品。然后，产生的过氧化氢在 SAN 催化下氧化 3,3'，5,5'-四甲基联苯胺，产生典型的蓝色产物。吸光度与半乳糖浓度之间的关系在 1-60μ m 范围内呈线性关系，检测限低至 0.23 μm。该策略可用于诊断半乳糖血症疾病和检测某些乳制品和其他商业产品中的半乳糖。DFT 计算阐明了 POD 样反应中 Cu 位点的高活性，并解释了 Cu-SAN 氧化酶样反应对 D-半乳糖的选择性

更新日期：2024-09-09

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