Quantum Sensing Unravels Antioxidant Efficacy Within PCL/Matrigel Skin Equivalents,Small

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Quantum Sensing Unravels Antioxidant Efficacy Within PCL/Matrigel Skin Equivalents
Small ( IF 13.0 ) Pub Date : 2024-09-09 , DOI: 10.1002/smll.202403729
Xixi Wu ₁ , Marcus Koch ₂ , Felipe P Perona Martínez ₁ , Romana Schirhagl ₁ , Małgorzata K Włodarczyk-Biegun _{3,

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Affiliation

Skin equivalents (SE) that recapitulate biological and mechanical characteristics of the native tissue are promising platforms for assessing cosmetics and studying fundamental biological processes. Methods to achieve SEs with well-organized structure, and ideal biological and mechanical properties are limited. Here, the combination of melt electrowritten PCL scaffolds and cell-laden Matrigel to fabricate SE is described. The PCL scaffold provides ideal structural and mechanical properties, preventing deformation of the model. The model consists of a top layer for seeding keratinocytes to mimic the epidermis, and a bottom layer of Matrigel-based dermal compartment with fibroblasts. The compressive modulus and the biological properties after 3-day coculture indicate a close resemblance with the native skin. Using the SE, a testing system to study the damage caused by UVA irradiation and evaluate antioxidant efficacy is established. The effectiveness of Tea polyphenols (TPs) and L-ascorbic acid (Laa) is compared based on free radical generation. TPs are demonstrated to be more effective in downregulating free radical generation. Further, T1 relaxometry is used to detect the generation of free radicals at a single-cell level, which allows tracking of the same cell before and after UVA treatment.

中文翻译：

量子传感揭示了 PCL/Matrigel 皮肤等效物中的抗氧化功效

概括天然组织的生物和机械特征的皮肤等效物（SE）是评估化妆品和研究基本生物过程的有前途的平台。获得具有组织良好的结构以及理想的生物和机械性能的 SE 的方法有限。在这里，描述了熔融电写 PCL 支架和载细胞 Matrigel 的组合来制造 SE。PCL 脚手架提供理想的结构和机械性能，防止模型变形。该模型由用于接种角质形成细胞以模拟表皮的顶层和带有成纤维细胞的基于 Matrigel 的真皮隔室的底层组成。共培养 3 天后的压缩模量和生物学特性表明与天然皮肤非常相似。使用 SE，建立了一个测试系统，用于研究 UVA 照射造成的损害并评估抗氧化功效。根据自由基的产生比较茶多酚（TPs）和 L-抗坏血酸（Laa）的有效性。TP 被证明在下调自由基产生方面更有效。此外，T1 弛豫测量法用于检测单细胞水平自由基的产生，从而可以跟踪 UVA 处理前后的同一细胞。

更新日期：2024-09-09

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