To achieve reliable detection of viral antigens, it is critical to develop of antifouling sensor arrays. Herein, we introduce a robust strategy for facile design and construction of hydrogel-based antifouling sensor arrays with a light-responsive bifunctional crosslinker. Upon UV light irradiation, the capture antibodies and blocking agents can be chemically conjugated onto the hydrogel interface to generate antifouling sensor arrays with desirable protein patterns with the aid of a hole-array mask. We demonstrated the antifouling sensor arrays for the quantitative detection of SARS-CoV-2 spike protein with satisfying sensitivity and high signal-to-background ratio. We further confirmed the prepared multiplexed sensor arrays for the detection of multiple viral proteins simultaneously in complex environments. Moreover, we achieved the quantitative detection of synthetic virus-like particles and SARS-CoV-2 pseudoviruses with satisfying detection performances. All these evidences suggested that the hydrogel-based antifouling sensor arrays holds great potential for quantitative analysis of viral antigens and virus particles in real applications.

中文翻译：

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光响应双功能交联剂可以轻松制备用于病毒抗原免疫检测的防污传感器阵列


为了实现病毒抗原的可靠检测，开发防污传感器阵列至关重要。在此，我们介绍了一种稳健的策略，可以使用光响应双功能交联剂轻松设计和构建基于水凝胶的防污传感器阵列。在紫外线照射下，捕获抗体和封闭剂可以化学结合到水凝胶界面上，借助孔阵列掩模生成具有所需蛋白质图案的防污传感器阵列。我们展示了用于定量检测 SARS-CoV-2 刺突蛋白的防污传感器阵列，具有令人满意的灵敏度和高信背景比。我们进一步证实了所制备的多重传感器阵列可用于在复杂环境中同时检测多种病毒蛋白。此外，我们还实现了合成病毒样颗粒和SARS-CoV-2假病毒的定量检测，检测性能令人满意。所有这些证据表明，基于水凝胶的防污传感器阵列在实际应用中对病毒抗原和病毒颗粒的定量分析具有巨大的潜力。