Hydrogen sulfide (H₂S) is both a biologically significant signaling molecule, and a harmful gas that is generated in the environment; thus, its detection and monitoring are required in range of biological and environmental systems. Most fluorescent probes for H₂S detection rely on single-detection fluorescence signals, making them vulnerable to interference from other species in complex microenvironment. In this study, we developed a ratiometric fluorogenic and colorimetric assay for H₂S detection, in which pyrene and naphthalimide fluorophores operate together to provide a selective and sensitive detection system. Pyrene maintains fluorescence (always ‘On’), while naphthalimide exhibits an ‘On-Off’ response through thiolysis with H₂S. By measuring the ratiometric fluorescence from these moieties, a self-calibrating response can be measured that is resistant to interference from other species in the microenvironments. The probe was used to measure H₂S generation from H₂S donor substrates and to monitor H₂S fluctuations within living cells through selective, ratiometric fluorescence changes and real-time, dual-channel fluorescence imaging.

中文翻译：

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用于活细胞和释放剂中 H2S 检测的自校正比率荧光测定


硫化氢 （H₂S） 既是一种具有生物学意义的信号分子，也是一种在环境中产生的有害气体;因此，在一系列生物和环境系统中需要对其进行检测和监测。大多数用于 H₂S 检测的荧光探针依赖于单次检测荧光信号，使其在复杂微环境中容易受到其他物种的干扰。在这项研究中，我们开发了一种用于 H₂S 检测的比率荧光和比色测定法，其中芘和萘酰亚胺荧光团共同作用以提供选择性和灵敏的检测系统。芘保持荧光（始终“开”），而萘苯二酰亚胺通过 H₂S 的硫解表现出“开-关”反应。通过测量这些基团的比率荧光，可以测量出对微环境中其他物种的干扰具有抵抗力的自校准响应。该探针用于测量 H₂S 供体底物的 H₂S 生成，并通过选择性、比率荧光变化和实时双通道荧光成像监测活细胞内的 H₂S 波动。