The acerola non-pomace, a by-product from the juice clarification step and rich in phenolic compounds and ascorbic acid, was investigated for its biological potential through tests. Extracts were obtained using hydrothermal extraction (HT) at 121°C as well as CO-expanded ethanol (GXL) at 40°C. The GXL extract contained 159 mg/g of ascorbic acid, while the HT extract showed 70 mg/g. Catechin was found only in the HT extract, and quercetin only in the GXL extract. Both extracts exhibited similar antiglycemic potential, but the GXL extract showed twice the neuroprotective potential against acetylcholinesterase (AChE, IC=302 μg/mL) and lipoxygenase (LOX, IC=227 μg/mL) activities. In human breast cancer cells (MDA-MB-231), the GXL extract demonstrated better antitumor potential with an IC of 100 μg/mL compared to 250 μg/mL for the HT extract.

中文翻译：

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水热萃取和气体膨胀液体萃取获得的针叶樱桃副产物提取物生物潜力的比较分析


针叶樱桃非果渣是果汁澄清步骤的副产品，富含酚类化合物和抗坏血酸，通过测试研究了其生物潜力。使用 121°C 的水热提取 (HT) 以及 40°C 的 CO 膨胀乙醇 (GXL) 获得提取物。 GXL 提取物含有 159 mg/g 的抗坏血酸，而 HT 提取物则含有 70 mg/g。儿茶素仅存在于 HT 提取物中，槲皮素仅存在于 GXL 提取物中。两种提取物都表现出相似的抗血糖潜力，但 GXL 提取物对乙酰胆碱酯酶（AChE，IC50=302 μg/mL）和脂氧合酶（LOX，IC5=227 μg/mL）活性的神经保护潜力是其两倍。在人乳腺癌细胞 (MDA-MB-231) 中，GXL 提取物表现出更好的抗肿瘤潜力，IC 为 100 μg/mL，而 HT 提取物的 IC 值为 250 μg/mL。