Lipid droplets (LDs) have recently garnered significant attention due to their pivotal roles in life and diseases. Thus, seeking suitable tools to visualize the dynamics of LDs and reveal their mysterious functions is particularly important. Organic fluorescent probes provide significant advantages in terms of excellent sensitivity, high selectivity, and remarkable spatial resolution for the in situ and real-time detection and monitoring of diverse biological species. This review summarizes recent progress on organic fluorescent probes for the dynamic imaging of LDs in biological applications. First, the design principles of fluorescent LD probes are briefly described. The subsequent sections present versatile applications involving the use of representative fluorescent LD probes for super-resolution imaging, analyte sensing, organelle interaction visualization, and theranostics. Finally, we discuss our understanding and opinions regarding further directions for the development of high-performance LD probes. This review provides new insights to advance our understanding of LDs and is expected to inspire the design of more intelligent and powerful fluorescent LD probes.

中文翻译：

---

用于可视化脂滴动态的多功能有机荧光探针


脂滴 （LDs） 因其在生活和疾病中的关键作用而最近引起了广泛关注。因此，寻找合适的工具来可视化 LD 的动力学并揭示其神秘功能尤为重要。有机荧光探针在出色的灵敏度、高选择性和卓越的空间分辨率方面具有显著的优势，可用于各种生物物种的原位和实时检测和监测。本文总结了有机荧光探针在生物应用中用于 LDs 动态成像的最新进展。首先，简要描述了荧光 LD 探针的设计原理。随后的章节介绍了多种应用，涉及使用代表性荧光 LD 探针进行超分辨率成像、分析物传感、细胞器相互作用可视化和治疗诊断学。最后，我们讨论了我们对高性能 LD 探针开发进一步方向的理解和看法。这篇综述为促进我们对 LD 的理解提供了新的见解，并有望激发更智能、更强大的荧光 LD 探针的设计。