New Utilization of Fraxinus mandshurica Leaves: As a Safe and Promising Natural Antioxidant,Journal of Agricultural and Food Chemistry

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New Utilization of Fraxinus mandshurica Leaves: As a Safe and Promising Natural Antioxidant
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-09 , DOI: 10.1021/acs.jafc.4c04961
Jianjin Guo _{1,

2} , Chi-Tang Ho ₃ , Naisheng Bai ₂

Affiliation

In this paper, an in-depth study on Fraxinus mandshurica (FM) was conducted, focusing on the chemical constituents, in vitro and in vivo antioxidant activities of flavonoids, acute oral toxicity testing, network pharmacology, and molecular docking in the leaves of FM. The in vitro antioxidant results revealed that the total flavonoid extract (TFE), kaempferol, quercetin, and rutin exhibited similar antioxidant activities, with TFE demonstrating significantly better scavenging ability against hydroxyl radical compared to the other flavonoids. Moreover, in vivo antioxidant findings indicated that TFE led to a significant increase in glutathione peroxidase and superoxide dismutase activities along with a decrease in malondialdehyde levels in the liver tissues of mice in an ethanol-induced oxidative stress model, outperforming quercetin. The acute oral toxicity test established 5000 mg/kg of bw as the LD₅₀ for TFE in rats. Through network pharmacological analysis, it was observed that all seven flavonoids in FM exhibited spontaneous binding to their respective key targets, reinforcing their potential antioxidant properties. Consequently, based on the experimental outcomes, TFE appears to be a safe and promising antioxidant source, indicating its potential as a new natural antioxidant resource.

中文翻译：

水曲柳叶的新用途：作为一种安全且有前景的天然抗氧化剂

本文对水曲柳（FM）进行了深入的研究，重点研究了水曲柳的化学成分、黄酮类化合物的体内外抗氧化活性、急性经口毒性试验、网络药理学以及水曲柳叶中的分子对接等。。体外抗氧化结果显示，总黄酮提取物（TFE）、山奈酚、槲皮素和芦丁表现出相似的抗氧化活性，其中TFE比其他黄酮类化合物表现出明显更好的羟自由基清除能力。此外，体内抗氧化研究结果表明，在乙醇诱导的氧化应激模型中，TFE 可显着增加谷胱甘肽过氧化物酶和超氧化物歧化酶活性，同时降低小鼠肝组织中的丙二醛水平，其表现优于槲皮素。急性经口毒性试验确定大鼠体内 TFE 的 LD ₅₀为 5000 mg/kg 体重。通过网络药理学分析，观察到 FM 中的所有七种黄酮类化合物均表现出与各自关键靶标的自发结合，增强了其潜在的抗氧化特性。因此，根据实验结果，TFE 似乎是一种安全且有前途的抗氧化剂来源，表明其作为新的天然抗氧化剂资源的潜力。

更新日期：2024-09-09

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