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In Situ and Rapid Toxicity Assessment of Air Pollution by Self-Assembly Passive Colonization Hydrogel
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2024-09-09 , DOI: 10.1021/acs.est.4c04807 Shuo Yang 1 , Mingliang Fang 1 , Ling Jin 2, 3 , Zhiwei Shao 1 , Xiang Zhang 1 , Yong Han 2 , Banghao Du 4 , Dayong Yang 5 , April Z Gu 6 , Yingjun Chen 1 , Dan Li 1 , Jianmin Chen 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2024-09-09 , DOI: 10.1021/acs.est.4c04807 Shuo Yang 1 , Mingliang Fang 1 , Ling Jin 2, 3 , Zhiwei Shao 1 , Xiang Zhang 1 , Yong Han 2 , Banghao Du 4 , Dayong Yang 5 , April Z Gu 6 , Yingjun Chen 1 , Dan Li 1 , Jianmin Chen 1
Affiliation
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Air pollution is a leading environmental health risk factor, and in situ toxicity assessment is urgently needed. Bacteria-based bioassays offer cost-effective and rapid toxicity assessments. However, the application of these bioassays for air toxicity assessment has been challenging, due to the instability of bacterial survival and functionality when directly exposed to air pollutants. Here, we developed an approach employing self-assembly passive colonization hydrogel (SAPCH) for in situ air toxicity assessment. The SAPCH features a core–shell structure, enabling the quantitatively immobilization of bacteria on its shell while continuously provides nutrients from its core. An antimicrobial polyelectrolyte layer between the core and shell confines bacteria to the air–liquid interface, synchronizing bacterial survival with exposure to air pollutants. The SAPCH immobilized a battery of natural and recombinant luminescent bacteria, enabling simultaneous detection of various toxicological endpoints (cytotoxicity, genotoxicity and oxidative stress) of air pollutants within 2 h. Its sensitivity was 3–5 orders of magnitude greater than that of traditional liquid-phase toxicity testing, and successfully evaluating the toxicity of volatile organic compounds and combustion smoke. This study presents a method for in situ, rapid, and economical toxicity assessment of air pollution, making a significant contribution to future air quality monitoring and control.
中文翻译：
自组装被动定植水凝胶对空气污染的原位和快速毒性评估
空气污染是一个主要的环境健康风险因素，迫切需要原位毒性评估。基于细菌的生物测定提供经济高效且快速的毒性评估。然而，由于细菌直接暴露于空气污染物时细菌存活和功能的不稳定性，这些生物测定法在空气毒性评估中的应用一直具有挑战性。在这里，我们开发了一种采用自组装被动定植水凝胶 （SAPCH） 进行原位空气毒性评估的方法。SAPCH 具有核壳结构，能够定量地将细菌固定在其壳上，同时不断从其核心提供营养。核心和外壳之间的抗菌聚电解质层将细菌限制在气液界面，使细菌存活与空气污染物暴露同步。SAPCH 固定了一组天然和重组发光细菌，能够在 2 小时内同时检测空气污染物的各种毒理学终点（细胞毒性、遗传毒性和氧化应激）。其灵敏度比传统液相毒性测试高 3-5 个数量级，并成功评估了挥发性有机化合物和燃烧烟雾的毒性。本研究提出了一种原位、快速和经济的空气污染毒性评估方法，为未来的空气质量监测和控制做出了重大贡献。
更新日期：2024-09-09
中文翻译：
自组装被动定植水凝胶对空气污染的原位和快速毒性评估
空气污染是一个主要的环境健康风险因素，迫切需要原位毒性评估。基于细菌的生物测定提供经济高效且快速的毒性评估。然而，由于细菌直接暴露于空气污染物时细菌存活和功能的不稳定性，这些生物测定法在空气毒性评估中的应用一直具有挑战性。在这里，我们开发了一种采用自组装被动定植水凝胶 （SAPCH） 进行原位空气毒性评估的方法。SAPCH 具有核壳结构，能够定量地将细菌固定在其壳上，同时不断从其核心提供营养。核心和外壳之间的抗菌聚电解质层将细菌限制在气液界面，使细菌存活与空气污染物暴露同步。SAPCH 固定了一组天然和重组发光细菌，能够在 2 小时内同时检测空气污染物的各种毒理学终点（细胞毒性、遗传毒性和氧化应激）。其灵敏度比传统液相毒性测试高 3-5 个数量级，并成功评估了挥发性有机化合物和燃烧烟雾的毒性。本研究提出了一种原位、快速和经济的空气污染毒性评估方法，为未来的空气质量监测和控制做出了重大贡献。
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