Bamboo, recognized for its rapid growth and versatility, is increasingly important in industrial applications. Succus Bambusae, a bamboo-derived product, has been extensively utilized as a traditional Chinese medicinal herb for its significant therapeutic effects on respiratory ailments such as cough and asthma for thousands of years. The escalating market demand for Succus Bambusae has led to a proliferation of preparation methods, which in turn poses considerable risks to its clinical application. The urgent need to investigate the quality differences and pharmacological activities among various Succus Bambusae products has become apparent. This study introduces a novel analytical approach that integrates plant metabolomics with network pharmacology and spectrum-effect relationship analysis. This comprehensive strategy was employed to discern the chemical variations and evaluate the efficacy differences in Succus Bambusae prepared by distinct methods, specifically fire-roasting and squeezing. Plant metabolomics was instrumental in identifying differential metabolites between the fire-roasted and squeezed Succus Bambusae, thereby establishing a chemical foundation for distinguishing between these two preparation techniques. Furthermore, network pharmacology was utilized to predict the mechanisms by which these differential metabolites exert their effects on cough and asthma, enabling the selection of key targets for the development of a cellular inflammation model. Additionally, the chemical profiles of both fire-roasted and squeezed Succus Bambusae were meticulously constructed. The spectrum-effect relationship between chemical components and the expression of inflammatory factor was analyzed using random forest regression model. The study successfully identified ten potential anti-inflammatory active components within Succus Bambusae. The holistic 'composition-activity-quality' strategy proposed herein offers a valuable framework for the quality assessment of not only Succus Bambusae but also other industrial and economic crops.

中文翻译：

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利用比较代谢组学、集成网络药理学和谱效关系方法揭示不同方法制备的竹汁的化学差异和药理活性


竹子以其快速生长和多功能性而闻名，在工业应用中日益重要。竹汁是一种竹子制品，数千年来因其对咳嗽、哮喘等呼吸系统疾病具有显着的治疗作用而被广泛用作传统中药材。市场对竹汁的需求不断增加，导致其制备方法不断增多，这也给其临床应用带来了相当大的风险。研究各种竹汁产品之间的质量差异和药理活性的迫切需要已经变得显而易见。这项研究引入了一种新颖的分析方法，将植物代谢组学与网络药理学和谱效关系分析相结合。采用这种综合策略来辨别化学变化并评估通过不同方法（特别是火烤和压榨）制备的竹汁的功效差异。植物代谢组学有助于识别火烤竹汁和压榨竹汁之间的差异代谢物，从而为区分这两种制备技术奠定了化学基础。此外，利用网络药理学来预测这些差异代谢物对咳嗽和哮喘发挥作用的机制，从而能够选择开发细胞炎症模型的关键靶标。此外，火烤竹汁和压榨竹汁的化学成分均经过精心构建。采用随机森林回归模型分析化学成分与炎症因子表达的谱效关系。 该研究成功鉴定了竹汁中十种潜在的抗炎活性成分。本文提出的整体“成分-活性-质量”策略不仅为竹汁而且也为其他工业和经济作物的质量评估提供了有价值的框架。