Kaempferol (KAE) has good biological activity, it is important to isolate kaempferol from tea. In this study, hydrophilic molecularly imprinted resin (MIRs) with specific selectivity and environmental friendliness were prepared in water phase using aminophenol formaldehyde resin as a carrier for adsorption of KAE. During the preparation process, deep eutectic solvents (DES) were used as co-solvents for pore makers and templates to improve the specific surface area of MIRs. DES replace the use of organic solvents and make the whole process green. Due to their high selectivity (imprinted factors =3.82) and aqueous phase compatibility, the MIRs can specifically recognize and extract KAE from aqueous phase. In comparison with reported adsorbent materials, MIRs have better specific ability to extract KAE and higher adsorption capacity (Q=603.43 mg/g). Mechanistic studies have demonstrated that the main forces involved in adsorption are hydrogen bonding and electrostatic attraction. A method combining solid phase extraction and high-performance liquid chromatography was developed for the determination of KAE in tea. The developed method showed good linearity (R=0.9992), lower limit of detection (0.29 μg/mL) and excellent relative standard deviation (RSD ≤ 3.9 %) The prepared MIRs proved to be a green and high adsorption capacity with promising applications in the purification and separation of alkaloids from natural products.

中文翻译：

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低共熔溶剂型亲水分子印迹树脂的制备用于茶叶中山奈酚的分离纯化


山奈酚(KAE)具有良好的生物活性，从茶叶中分离山奈酚具有重要意义。本研究以氨基酚甲醛树脂为载体，在水相中制备了具有特异性选择性且环境友好的亲水性分子印迹树脂（MIR），用于吸附KAE。在制备过程中，使用低共熔溶剂（DES）作为造孔剂和模板的共溶剂，以提高 MIR 的比表面积。 DES取代有机溶剂的使用，使整个过程绿色化。由于其高选择性（印迹因子 = 3.82）和水相兼容性，MIR 可以特异性识别并从水相中提取 KAE。与已报道的吸附材料相比，MIRs具有更好的提取KAE的比能力和更高的吸附容量（Q=603.43 mg/g）。机理研究表明，参与吸附的主要作用力是氢键和静电引力。建立了固相萃取与高效液相色谱联用测定茶叶中KAE的方法。所开发的方法具有良好的线性（R=0.9992）、检测下限（0.29 μg/mL）和优异的相对标准偏差（RSD ≤ 3.9 %）。所制备的 MIR 被证明是一种绿色且高吸附容量的材料，在工业领域具有广阔的应用前景。从天然产物中纯化和分离生物碱。