stands out as a highly valuable tree species crucial for industries and ecological restoration projects in tropical and subtropical regions, owing to its exceptional salt resistance. However, its growth is hampered in cold and drought-prone areas. Understanding the genetic basis for abiotic stress tolerance is imperative for enhancing stress-resistant breeding efforts of . However, the CBF genes are renowned for their pivotal roles in cold response and broad resilience to other adverse environments, such as drought, and high salinity. In this study, five CeCBFs were identified from the genome of . CeCBF1 and CeCBF3 belong to Clade I group, which diverged early from DREBIII members, while CeCBF2, CeCBF4, and CeCBF5 are classified into Clade II, a group innovated from Clade I. These genes lack introns in their sequences. The proteins encoded by CeCBFs feature classic AP2 domains and CBF signature sequences, localize in the nucleus and exhibit transactivation activity. Additionally, stress-related, abscisic acid, and jasmonic acid responsive elements are present on the CeCBFs promoters. Expression analysis revealed that CeCBFs were prodominantly induced by cold, drought, and salinity treatments, except for , which showed repression specifically under low temperature. Moreover, , , and were inhibited by abscisic acid, while showed reduced transcript levels in response to methyl jasmonate treatment. Furthermore, overexpression of enhanced tolerance to low temperature, depriving of water, and salt by up-regulating stress-responsive genes expression and promoting proline accumulation, albeit with a trade-off in growth and productivity. Hence, CeCBFs emerge as potential regulators contributing significantly to stress tolerance mechanisms in .

中文翻译：

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木麻黄中 C 重复结合因子 (CBF) 的鉴定和非生物胁迫响应的表达模式以及 CeCBF4 的过表达可增加拟南芥的胁迫耐受性


由于其卓越的耐盐性，它成为对热带和亚热带地区的工业和生态恢复项目至关重要的高价值树种。然而，它的生长在寒冷和干旱多发地区受到阻碍。了解非生物胁迫耐受性的遗传基础对于加强抗逆育种工作至关重要。然而，CBF 基因因其在寒冷反应和对其他不利环境（如干旱和高盐度）的广泛恢复能力中的关键作用而闻名。在这项研究中，从 的基因组中鉴定出了 5 个 CeCBF。 CeCBF1和CeCBF3属于Clade I类群，该类群较早从DREBIII成员分化而来，而CeCBF2、CeCBF4和CeCBF5属于Clade II类，是从Clade I创新而来的类群。这些基因在其序列中缺乏内含子。 CeCBF 编码的蛋白质具有经典的 AP2 结构域和 CBF 特征序列，定位于细胞核并表现出反式激活活性。此外，CeCBF 启动子上还存在应激相关的脱落酸和茉莉酸响应元件。表达分析表明，CeCBF 主要受寒冷、干旱和盐度处理诱导，但在低温下表现出特异性抑制。此外， 、 、 和 被脱落酸抑制，而响应茉莉酸甲酯处理而表现出转录水平降低。此外，过度表达通过上调应激反应基因表达和促进脯氨酸积累来增强对低温、缺水和盐的耐受性，尽管会在生长和生产力方面做出权衡。因此，CeCBF 作为潜在的监管者而出现，对压力耐受机制做出了重大贡献。