DNA Origami Vesicle Sensors with Triggered Single-Molecule Cargo Transfer,Angewandte Chemie International Edition

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DNA Origami Vesicle Sensors with Triggered Single-Molecule Cargo Transfer
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2024-09-09 , DOI: 10.1002/anie.202408295
Ece Büber ₁ , Renukka Yaadav ₂ , Tim Schröder ₂ , Henri G Franquelim ₃ , Philip Tinnefeld ₄

Affiliation

This study presents a DNA origami-based biosensor for precise lipid vesicle detection and cargo delivery using single-molecule Fluorescence Resonance Energy Transfer (smFRET). The hydrophobic ATTO647N-modified single-stranded DNA (ssDNA) leash on the sensor detects vesicle binding via FRET changes and delivers molecular cargo through triggered strand displacement reaction. This platform offers innovative applications in biosensing and controllable stoichiometric loading of vesicles.

中文翻译：

具有触发单分子货物转移的 DNA 折纸囊泡传感器

本研究提出了一种基于 DNA 折纸的生物传感器，用于使用单分子荧光共振能量转移（smFRET）进行精确的脂质囊泡检测和货物递送。传感器上的疏水 ATTO647N 修饰的单链 DNA （ssDNA）皮带通过 FRET 变化检测囊泡结合，并通过触发的链置换反应递送分子货物。该平台在囊泡的生物传感和可控化学计量负载方面提供了创新应用。

更新日期：2024-09-09

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