The efficacy of fluorescence-guided surgery in facilitating the real-time delineation of tumours depends on the optical contrast of tumour tissue over healthy tissue. Here we show that CJ215—a commercially available, renally cleared carbocyanine dye sensitive to apoptosis, and with an absorption and emission spectra suitable for near-infrared fluorescence imaging (wavelengths of 650–900 nm) and shortwave infrared (SWIR) fluorescence imaging (900–1,700 nm)—can facilitate fluorescence-guided tumour screening, tumour resection and the assessment of wound healing. In tumour models of either murine or human-derived breast, prostate and colon cancers and of fibrosarcoma, and in a model of intraperitoneal carcinomatosis, imaging of CJ215 with ambient light allowed for the delineation of nearly all tumours within 24 h after intravenous injection of the dye, which was minimally taken up by healthy organs. At later timepoints, CJ215 provided tumour-to-muscle contrast ratios up to 100 and tumour-to-liver contrast ratios up to 18. SWIR fluorescence imaging with the dye also allowed for quantifiable non-contact wound monitoring through commercial bandages. CJ215 may be compatible with existing and emerging clinical solutions.

荧光引导手术在促进肿瘤实时描绘方面的疗效取决于肿瘤组织与健康组织的光学对比度。在这里，我们展示了 CJ215——一种对细胞凋亡敏感的市售、肾清除的羰菁染料，具有适用于近红外荧光成像（波长为 650-900 nm）和短波红外 （SWIR） 荧光成像（900-1,700 nm）的吸收和发射光谱——可以促进荧光引导的肿瘤筛查、肿瘤切除和伤口愈合评估。在小鼠或人源性乳腺癌、前列腺癌和结肠癌以及纤维肉瘤的肿瘤模型中，以及在腹膜内癌病模型中，CJ215 在环境光下成像允许在静脉注射染料后 24 小时内描绘几乎所有肿瘤，其被健康器官吸收最少。在较晚的时间点，CJ215 的肿瘤与肌肉对比比高达 100，肿瘤与肝脏对比比高达 18。使用染料进行 SWIR 荧光成像还允许通过商业绷带进行可量化的非接触式伤口监测。CJ215 可能与现有和新兴的临床解决方案兼容。