The aim of this study was to investigate the effects of various concentrations of antioxidants, including butyl hydroxy anisd (BHA), butylated hydroxytoluene (BHT), fulvic acid (FA), melatonin (MT), glycine betaine (GB) and putrescine (Put), on growth and lipid synthesis of microalgae under low-temperature (15 ℃). Changes in biochemical indicators, reactive oxygen species (ROS) level, glutathione (GSH) content and antioxidant enzyme activities were also studied. The results indicated that the maximum biomass concentration (1.3 g/L) and lipid productivity (75.3 ± 5.8 mg/L d-1) were achieved under 100 μM MT and 1 μM GB, respectively. Moreover, antioxidants were able to increase the GSH and antioxidant enzymes activities in algal cells under low-temperature stress. This study was enlightening for the utilization of antioxidants to improve the resistance to low-temperature stress and lipid production in microalgae, and provided a theoretical basis for the application of microalgae for lipid accumulation in cold regions.

中文翻译：

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抗氧化剂通过调节活性氧来改善脂质产生和抗氧化防御，从而缓解微藻中的低温胁迫


本研究旨在探讨不同浓度的抗氧化剂，包括丁基羟基茴香 （BHA）、丁基羟基甲苯 （BHT）、黄腐酸 （FA）、褪黑激素 （MT）、甘氨酸甜菜碱 （GB） 和腐胺 （Put），对低温 （15 °C） 下微藻生长和脂质合成的影响。还研究了生化指标、活性氧 （ROS） 水平、谷胱甘肽 （GSH） 含量和抗氧化酶活性的变化。结果表明，在 100 μM MT 和 1 μM GB 下，分别达到最大生物量浓度 （1.3 g/L） 和脂质产率 （75.3 ± 5.8 mg/L d-1）。此外，抗氧化剂能够在低温胁迫下增加藻类细胞中的 GSH 和抗氧化酶活性。本研究为利用抗氧化剂提高微藻对低温胁迫的抵抗力和脂质产生具有启发性，为微藻在寒冷地区脂质积累的应用提供了理论依据。