Aflatoxins, zearalenone and its metabolites, as representative hazard mycotoxins cause adverse effects on food safety and human health. Developing a sensitive and reliable extraction and detection method is of great importance for monitoring their residue and exposure levels. In contrast to traditional trial-and-error selection steps, 4,4′,4″-(1,3,5-triazine-2,4,6-triyl) trianiline covalent-bonding with 2,5-dihydroxyterephthalaldehyde, namely TAPT-OH-COF was screened as a potential adsorbent utilizing density functional theory calculations prior to the synthesis procedure. After experimental verification, magnetic TAPT-OH-COFs were prepared, characterized and applied for the extraction of aflatoxins, zearalenone and its metabolites from food and biological samples, coupled with high-performance liquid chromatography tandem mass spectrophy detection. Under the optimal conditions, the developed method exhibited low limits of quantification (0.05–0.50 μg/kg), satisfactory recoveries (75.8 %–110.9 %) and good precision with intraday and interday relative standard deviations (RSDs) not exceeding 12.2 %. This study may provide great potential for the selection of candidate adsorbents for multi-mycotoxins extraction from complex samples.

中文翻译：

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合理选择 4,4′，4“-（1,3,5-三嗪-2,4,6-三基）三苯胺基共价有机框架作为吸附剂，用于有效共萃取食品和生物样品中的黄曲霉毒素、玉米赤霉烯酮及其代谢物


黄曲霉毒素、玉米赤霉烯酮及其代谢物作为代表性的危害霉菌毒素，对食品安全和人类健康造成不利影响。开发一种灵敏可靠的提取和检测方法对于监测其残留物和暴露水平非常重要。与传统的试错选择步骤相比，4,4′，4“-（1,3,5-三嗪-2,4,6-三基）三苯胺与 2,5-二羟基对苯二甲醛，即 TPT-OH-COF在合成程序之前利用密度泛函理论计算筛选为潜在的吸附剂。经实验验证，制备磁性 TAPT-OH-COFs，对其进行表征，并应用于食品和生物样品中黄曲霉毒素、玉米赤霉烯酮及其代谢物的提取，并结合高效液相色谱串联质谱检测。在最佳条件下，所开发的方法表现出低定量限 （0.05–0.50 μg/kg）、令人满意的回收率 （75.8 %–110.9 %） 和良好的精密度，日内和日间相对标准偏差 （RSD） 不超过 12.2 %。本研究可能为从复杂样品中提取多种霉菌毒素的候选吸附剂的选择提供巨大潜力。