Antibiotic resistance genes (ARGs) persistence and potential harm have become more widely recognized in the environment due to its fast-paced research. However, the bibliometric review on the detection, research hotspot, and development trend of environmental ARGs has not been widely conducted. It is essential to provide a comprehensive overview of the last 30 years of research on environmental ARGs to clarify the changes in the research landscape and ascertain future prospects. This study presents a visualized analysis of data from the Web of Science to enhance our understanding of ARGs. The findings indicate that solid-phase extraction provides a reliable method for extracting ARG. Technological advancements in commercial kits and microfluidics have facilitated the efficacy of ARGs extraction with significantly reducing processing times. PCR and its derivatives, DNA sequencing, and multi-omics technology are the prevalent methodologies for ARGs detection, enabling the expansion of ARG research from individual strains to more intricate microbial communities in the environment. Furthermore, due to the development of combination, hybridization and mass spectrometer technologies, considerable advancements have been achieved in terms of sensitivity and accuracy as well as lowering the cost of ARGs detection. Currently, high-frequency terms such as “Antibiotic Resistance, Antibiotics, and Metagenomics” are the center of attention for study in this area. Prominent topics include the investigation of anthropogenic impacts on environmental resistance, as well as the dynamics of migration, dissemination, and adaptation of environmental ARGs, etc. The research on environmental ARGs has made significant advancements in the fields of “Microbiology” and “Biotechnology Applied Microbiology”. Over the past decade, there has been a notable increase in the fields of “Environmental Sciences Ecology” and “Engineering” with a similar growth trend observed in “Water Resources”. These three domains are expected to continue driving extensive study within the realm of environmental ARGs.

中文翻译：

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环境抗生素耐药基因检测最新进展及研究热点：从基因到生态系统


由于快速的研究，抗生素抗性基因（ARG）的持久性和潜在危害在环境中已得到更广泛的认识。然而，关于环境ARGs的检测、研究热点和发展趋势的文献综述尚未广泛开展。有必要对过去 30 年环境 ARGs 研究进行全面概述，以阐明研究格局的变化并确定未来的前景。这项研究对来自 Web of Science 的数据进行了可视化分析，以增强我们对 ARG 的理解。研究结果表明固相萃取为提取 ARG 提供了一种可靠的方法。商业试剂盒和微流体技术的进步提高了 ARG 提取的功效，并显着缩短了处理时间。 PCR 及其衍生物、DNA 测序和多组学技术是 ARG 检测的流行方法，使 ARG 研究从单个菌株扩展到环境中更复杂的微生物群落。此外，由于组合、杂交和质谱技术的发展，在灵敏度和准确性以及降低ARG检测成本方面取得了相当大的进步。目前，“抗生素耐药性、抗生素和宏基因组学”等高频术语是该领域研究的焦点。突出的主题包括研究人为对环境抵抗力的影响，以及环境抗性基因的迁移、传播和适应动态等。 环境ARGs的研究在“微生物学”和“生物技术应用微生物学”领域取得了重大进展。过去十年，“环境科学、生态学”和“工程”领域的增长显着，“水资源”领域也有类似的增长趋势。预计这三个领域将继续推动环境 ARG 领域的广泛研究。