Isolation and Identification of Human Gut Bacteria Capable of Converting Curcumin to Its Hydrogenated Metabolites,Journal of Agricultural and Food Chemistry

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Isolation and Identification of Human Gut Bacteria Capable of Converting Curcumin to Its Hydrogenated Metabolites
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-06 , DOI: 10.1021/acs.jafc.4c03828
Minna Luo ₁ , Siu Wong ₁ , Pongpol Thanuphol ₁ , Hengjun Du ₁ , Yanhui Han ₁ , Margaret Lin ₁ , Xiaojing Guo ₁ , Tyler D Bechtel ₁ , John G Gibbons ₁ , Hang Xiao ₁

Affiliation

Curcumin is widely recognized for its health benefits, though the role of gut microbiota in its metabolic transformation was not well studied. In this study, bacterial strains capable of metabolizing curcumin were isolated from human stool samples. Using 16S rRNA and whole-genome sequencing, two novel strains (Clostridium butyricum UMA_cur1 and Escherichia coli UMA_cur2) were identified. In addition, the metabolic products were analyzed using liquid chromatography-mass spectrometry. These strains efficiently converted curcumin into dihydro-curcumin (DHC) and tetrahydro-curcumin (THC). Notably, E. coli UMA_cur2 also produced hexahydro-curcumin (HHC) and octahydro-curcumin (OHC), marking the first identification of a strain capable of such transformations. The absence of the YncB gene (typically involved in curcumin conversion) in C. butyricum UMA_cur1 suggests an alternative metabolic pathway. Curcumin metabolism begins during the stationary growth phase, indicating that it is not crucial for primary growth functions. Furthermore, E. coli UMA_cur2 produced these metabolites sequentially, starting with DHC and THC and progressing to HHC and OHC. These findings identified two novel strains that can metabolize curcumin to hydrogenated metabolites, which enhance our understanding of the interaction between curcumin and gut microbiota.

中文翻译：

能够将姜黄素转化为其氢化代谢物的人类肠道细菌的分离和鉴定

姜黄素因其健康益处而被广泛认可，但肠道微生物群在其代谢转化中的作用尚未得到充分研究。在这项研究中，从人类粪便样本中分离出了能够代谢姜黄素的细菌菌株。利用 16S rRNA 和全基因组测序，鉴定出两种新菌株（丁酸梭菌UMA_cur1 和大肠杆菌UMA_cur2）。此外，使用液相色谱-质谱法分析代谢产物。这些菌株有效地将姜黄素转化为二氢姜黄素（DHC）和四氢姜黄素（THC）。值得注意的是，大肠杆菌UMA_cur2 还产生六氢姜黄素 (HHC) 和八氢姜黄素 (OHC)，这标志着首次鉴定出能够进行此类转化的菌株。 C. butyricum UMA_cur1 中缺乏 YncB 基因（通常参与姜黄素转化）表明存在替代代谢途径。姜黄素代谢在稳定生长阶段开始，表明它对于初级生长功能并不重要。此外，大肠杆菌UMA_cur2 依次产生这些代谢物，从 DHC 和 THC 开始，逐渐产生 HHC 和 OHC。这些发现确定了两种可以将姜黄素代谢为氢化代谢物的新菌株，这增强了我们对姜黄素与肠道微生物群之间相互作用的理解。

更新日期：2024-09-06

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