Using CO 2 as the primary feedstock offers the potential for high-value utilization of CO 2 while forging sustainable pathways for producing valuable natural products, such as l -tyrosine. Cascade catalysis is a promising approach but limited by stringent purity demands of nexus molecules. We developed an abiotic/biotic cascade catalysis using blended nexus molecules for l -tyrosine synthesis. Specifically, we begin by constructing a solid-state reactor to reduce CO 2 electrochemically, yielding a mixture of acetic acid and ethanol, which serves as the blended nexus molecules. Subsequently, we use genetic engineering to introduce an ethanol utilization pathway and a tyrosine producing pathway to Escherichia coli to facilitate l -tyrosine production. The ethanol pathway synergistically cooperated with the acetic acid pathway, boosting l -tyrosine production rate (nearly five times higher compared to the strain without ethanol utilization pathway) and enhancing carbon efficiency. Our findings demonstrate that using blended nexus molecules could potentially offer a more favorable strategy for the cascade catalysis aimed at producing valuable natural products.

中文翻译：

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混合连接分子促进 CO 2 向 L-酪氨酸的转化


使用CO 2 作为主要原料提供了CO 2 高价值利用的潜力，同时开辟了生产有价值的天然产品（例如l-酪氨酸）的可持续途径。级联催化是一种有前途的方法，但受到连接分子严格纯度要求的限制。我们开发了一种使用混合连接分子进行 L-酪氨酸合成的非生物/生物级联催化。具体来说，我们首先构建一个固态反应器，以电化学方式还原CO 2 ，​​产生乙酸和乙醇的混合物，作为混合连接分子。随后，我们利用基因工程将乙醇利用途径和酪氨酸生产途径引入大肠杆菌，以促进L-酪氨酸的生产。乙醇途径与乙酸途径协同配合，提高了L-酪氨酸产率（比没有乙醇利用途径的菌株高出近五倍）并提高了碳效率。我们的研究结果表明，使用混合联结分子可能为旨在生产有价值的天然产物的级联催化提供更有利的策略。