Understanding the phenotypic and transcriptional signature of immunoglobulin E (IgE)–producing cells is fundamental to plasma cell (PC) biology and development of therapeutic interventions for allergy. Here, using a mouse model of intranasal house dust mite (HDM) exposure, we showed that short-lived IgE PCs emerge in lung draining lymph nodes (dLNs) during early exposure (<3 weeks) and long-lived IgE PCs accumulate in the bone marrow (BM) with prolonged exposure (>7 weeks). IgE PCs had distinct surface and gene expression profiles in these different tissues compared with other Ig isotypes. IgE BMPCs up-regulated genes associated with prosurvival and BM homing, whereas IgE dLN PCs expressed genes associated with recent class switching and differentiation. IgE PCs also exhibited higher expression of endoplasmic reticulum (ER) stress and protein coding genes and higher antibody secretion rate when compared with IgG1. Overall, this study highlights the unique developmental path and transcriptional signature of short-lived and long-lived IgE PCs.

中文翻译：

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IgE 浆细胞在转录和功能上与其他同种型不同


了解免疫球蛋白 E (IgE) 产生细胞的表型和转录特征是浆细胞 (PC) 生物学和过敏治疗干预措施开发的基础。在这里，使用鼻内屋尘螨 (HDM) 暴露的小鼠模型，我们发现在早期暴露期间（<3 周），短寿命的 IgE PC 出现在肺引流淋巴结 (dLN) 中，而长寿命的 IgE PC 则在肺引流淋巴结 (dLN) 中积累。长期暴露（>7 周）的骨髓 (BM)。与其他 Ig 同种型相比，IgE PC 在这些不同组织中具有不同的表面和基因表达谱。 IgE BMPC 上调与促生存和 BM 归巢相关的基因，而 IgE dLN PC 表达与近期类别转换和分化相关的基因。与 IgG1 相比，IgE PC 还表现出更高的内质网应激和蛋白质编码基因表达以及更高的抗体分泌率。总体而言，这项研究强调了短寿命和长寿命 IgE PC 的独特发育路径和转录特征。