Metagenomic sequencing technology was applied to evaluates the microbial diversity, functional activity, and synergistic relationships during the anaerobic fermentation process of landfill leachate and weathered coal, aiming to assess the key metabolic pathways in the combined anaerobic fermentation process of leachate and weathered coal. The results indicate that co-fermentation significantly enhances the production of biogenic methane. Furthermore, co-fermentation promotes the abundance of Paracoccus that involved in the degradation of organic pollutants, and enriches methane-producing archaea such as Methanothrix and Methanoculleus. Significant increases in carbohydrate enzymes such as lignin-degrading enzyme AAs, as well as GH2, GH43, GT4. The relative abundance of genes related to toluene degradation in co-anaerobic fermentation is 2.5 times and 1.3 times that of singular weathered coal and landfill leachate, respectively. The addition of leachate promotes the metabolic pathway of acetate conversion to methane. This research provides mechanistic studies on the treatment of waste leachate and weathered coal, and provides new ideas for environmental protection and clean energy.

中文翻译：

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风化煤中厌氧发酵用于生物甲烷生产的宏基因组学特征，垃圾渗滤液增强


应用宏基因组测序技术评价垃圾渗滤液和风化煤厌氧发酵过程中的微生物多样性、功能活性和协同关系，旨在评价渗滤液和风化煤联合厌氧发酵过程中的关键代谢途径。结果表明，共发酵显着促进了生物甲烷的产生。此外，共发酵促进了参与有机污染物降解的副球菌的丰度，并丰富了产生甲烷的古细菌，如甲烷丝菌和甲烷菌。碳水化合物酶（如木质素降解酶 AAs）以及 GH2、GH43、GT4 的显著增加。共厌氧发酵中与甲苯降解相关的基因的相对丰度分别为单一风化煤和垃圾渗滤液的 2.5 倍和 1.3 倍。渗滤液的添加促进了乙酸盐转化为甲烷的代谢途径。本研究为废渗滤液和风化煤的处理提供了机理研究，为环境保护和清洁能源提供了新思路。