This study investigated the toxicological effects and mechanisms of cadmium (Cd) (5 and 50 μg/L) and selenium (Se) (3 and 30 μg/L) at environmentally relevant concentrations on the gills and digestive glands of clams . Results indicated that Cd and Se could tissue-specifically impact osmoregulation, energy metabolism, and synaptic transmission in the gills and digestive glands of clams. After exposure to 50 μg/L Cd, the digestive glands of clams up-regulated the expression of and for detoxification. Clam digestive glands exposed to 3 μg/L Se up-regulated the expression of and to alleviate oxidative stress, and down-regulated the expression of to reduce the conversion of inorganic Se. Additionally, the interaction mode between Cd and Se largely depended on their molar ratio, with a ratio of 11.71 (50 μg/L Cd + 3 μg/L Se) demonstrated to be particularly harmful, as manifested by significantly more lesions, oxidative stress, and detoxification demand in clams than those exposed to Cd or Se alone. Collectively, this study revealed the complex interaction patterns and mechanisms of Cd and Se on clams, providing a reference for exploring their single and combined toxicity.

中文翻译：

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菲律宾蛤仔体内镉和硒的组合积累、应激反应、解毒和突触传递效应


本研究调查了环境相关浓度的镉 (Cd)（5 和 50 μg/L）和硒 (Se)（3 和 30 μg/L）对蛤的鳃和消化腺的毒理学效应和机制。结果表明，镉和硒可以组织特异性影响蛤的鳃和消化腺的渗透调节、能量代谢和突触传递。 50 μg/L Cd暴露后，蛤消化腺上调 和 的表达，进行解毒。 3 μg/L Se处理后的蛤消化腺上调 和 的表达以减轻氧化应激，下调 的表达以减少无机硒的转化。此外，Cd和Se之间的相互作用模式很大程度上取决于它们的摩尔比，摩尔比为11.71（50 μg/L Cd + 3 μg/L Se）被证明特别有害，表现为明显更多的病变、氧化应激、蛤蜊的解毒需求高于单独接触镉或硒的蛤蜊。总的来说，本研究揭示了Cd和Se对蛤类复杂的相互作用模式和机制，为探索它们的单一和组合毒性提供了参考。