Chemiluminescence (CL) bioassay is one of the most advanced and used detection method in clinical diagnosis and biomedical research because of the advantages of low background, easy operation, and wide-field imaging without a light source or microscope. The luminol/hydrogen peroxide/horseradish peroxidase (luminol/H2O2/HRP) system is the most popular CL system, but its application in high-throughput imaging detection is challenged due to its low luminescence efficiency and flash-type emission which is difficult in ensuring the reproducibility and consistency of detection results.

中文翻译：

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基于环糊精超分子增强剂的辉光型鲁米诺化学发光


化学发光 （CL） 生物测定法是临床诊断和生物医学研究中最先进和最常用的检测方法之一，因为它具有低背景、易于操作、无需光源或显微镜即可进行宽场成像等优点。鲁米诺/过氧化氢/辣根过氧化物酶 （luminol/H2O2/HRP） 系统是目前最流行的 CL 系统，但由于其发光效率低和闪光型发射难以保证检测结果的可重复性和一致性，其在高通量成像检测中的应用受到挑战。