TRY-NAD metabolic network includes TRY (tryptophan), 5-HT (5-hydroxytryptamine), KYN (kynurenine), and NAD (nicotinamide adenine dinucleotide) pathway, which plays a significant role in neurological diseases and ageing. It is important to monitor these metabolites for studying the pathological anatomy of disease and treatment of responses evaluation. Although previous studies have reported quantitative methods for several metabolites in the network, the bottlenecks of simultaneously quantifying the whole metabolic network are their similar structures, diverse physico-chemical properties, and instability. Standardized protocols for the whole metabolic network are still missing, which hinders the in-depth study of TRY-NAD metabolic network in laboratory research and clinical screening.

中文翻译：

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一种快速可靠的靶向 LC-MS/MS 方法，用于定量分析睡眠剥夺小鼠组织和血液中色氨酸-NAD 代谢网络紊乱


TRY-NAD 代谢网络包括 TRY（色氨酸）、5-HT（5-羟色胺）、KYN（犬尿氨酸）和 NAD（烟酰胺腺嘌呤二核苷酸）通路，在神经系统疾病和衰老中起重要作用。监测这些代谢物对于研究疾病的病理解剖学和反应评估的治疗非常重要。尽管以前的研究已经报道了网络中几种代谢物的定量方法，但同时量化整个代谢网络的瓶颈是它们相似的结构、不同的物理化学性质和不稳定性。整个代谢网络的标准化方案仍然缺失，这阻碍了 TRY-NAD 代谢网络在实验室研究和临床筛选中的深入研究。