Excessive use of veterinary drugs causes severely environmental pollution and agricultural pollution, and poses great threat to human health. A simple method for the rapid, highly sensitive, and on-site monitoring of veterinary drug residues in complex samples remains lacking. In this study, we propose a catalytically enhanced colorimetric lateral flow immunoassay (LFA) based on a novel core–satellite-structured magnetic nanozyme (Fe–Au@Pt) that can simultaneously and quantitatively detect three common veterinary drugs, namely, gentamicin (GM), streptomycin (STR), and clenbuterol (CLE), within a short testing time (<30 min). The Fe–Au@Pt nanozyme was simply prepared through the self-assembly of numerous Au@Pt nanoparticles on a large FeO core via electrostatic adhesion, which exhibited the advantages of high peroxidase-like activity, strong magnetic responsiveness, and multiple catalytic sites. Under the dual-signal amplification effect of magnetic enrichment and catalytic enhancement, the proposed nanozyme-LFA allowed the multiplex detection of STR, CLE, and GM with detection limits of 10.1, 6.3, and 1.1 pg/mL, respectively. The developed Fe–Au@Pt–LFA achieves direct, simultaneous, and accurate detection of three target drugs in food samples (honey, milk, and pork). The proposed assay shows great potential for application in the real-time monitoring of small-molecule pollutants in complex environment.

中文翻译：

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核心卫星结构磁性纳米酶能够在侧流免疫分析中对多种药物残留进行超灵敏比色检测


兽药的过量使用造成严重的环境污染和农业污染，对人类健康造成极大威胁。目前仍缺乏一种快速、高灵敏度、现场监测复杂样品中兽药残留的简单方法。在本研究中，我们提出了一种基于新型核心卫星结构磁性纳米酶（Fe-Au@Pt）的催化增强比色侧流免疫分析（LFA），可以同时定量检测三种常见兽药，即庆大霉素（GM） )、链霉素 (STR) 和克伦特罗 (CLE)，在很短的测试时间内（<30 分钟）。 Fe-Au@Pt纳米酶是通过静电粘附在大的Fe3O核心上自组装大量Au@Pt纳米颗粒而简单制备的，具有高过氧化物酶活性、强磁响应性和多个催化位点的优点。在磁富集和催化增强的双重信号放大作用下，所提出的纳米酶-LFA可以多重检测STR、CLE和GM，检测限分别为10.1、6.3和1.1 pg/mL。所开发的Fe-Au@Pt-LFA实现了食品样品（蜂蜜、牛奶和猪肉）中三种目标药物的直接、同步、准确检测。该方法在复杂环境中小分子污染物的实时监测中显示出巨大的应用潜力。