Visualization of Diabetes Progression by an Activatable NIR-IIb Luminescent Probe,Analytical Chemistry

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Visualization of Diabetes Progression by an Activatable NIR-IIb Luminescent Probe
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-06 , DOI: 10.1021/acs.analchem.4c02629
Yong Wan ₁ , Zhi Guo ₁ , Zhengjun Wu ₁ , Tao Liang ₁ , Zhen Li ₁

Affiliation

Developing NIR-IIb luminescence probes with rapid visualization and a high penetration depth is essential for diabetes research. Combining a sensitizing switch with lanthanide-doped nanoparticles (LnNPs) has been employed to fabricate the NIR-IIb probes. However, these probes mainly adopt heptamethine cyanine dye as the antenna, and the NIR-IIb signal is activated by inhibiting the photoinduced electron transfer (PET) of the dye. Due to limited recognition units, this strategy makes many biomolecules undetectable, such as cysteine (Cys), which is closely related to diabetes. Herein, in this article, hemicyanine dye, NFL-OH, was verified as a new antenna to sensitize NIR-IIb emission from LnNPs. Unlike traditional cyanine dyes, hemicyanine’s fluorescence intensity can also be modulated by intramolecular charge transfer (ICT), thereby expanding the range of detectable targets for NIR-IIb probes based on sensitization mechanism. Through switching the hemicyanine-sensitized NIR-IIb emission, we successfully fabricated an NFL-Cys-LnNPs’ nanoprobe, which can effectively monitor Cys concentration in the liver of diabetic mice during diabetes progression and evaluate the efficacy of diabetic drugs. Our work not only presents an excellent tool for Cys imaging but also introduces new concepts for designing NIR-IIb probes.

中文翻译：

通过可激活的 NIR-IIb 发光探针可视化糖尿病进展

开发具有快速可视化和高穿透深度的 NIR-IIb 发光探针对于糖尿病研究至关重要。将敏化开关与镧系元素掺杂纳米粒子 (LnNP) 相结合已用于制造 NIR-IIb 探针。然而，这些探针主要采用七次甲基花青染料作为天线，通过抑制染料的光致电子转移（PET）来激活NIR-IIb信号。由于识别单元有限，这种策略使得许多生物分子无法检测到，例如与糖尿病密切相关的半胱氨酸（Cys）。本文中，半花青染料 NFL-OH 被验证为一种新的天线，可以敏化 LnNP 的 NIR-IIb 发射。与传统的花青染料不同，半花青的荧光强度还可以通过分子内电荷转移（ICT）进行调节，从而扩大了基于敏化机制的NIR-IIb探针的可检测目标范围。通过切换半花青敏化的NIR-IIb发射，我们成功制备了NFL-Cys-LnNPs纳米探针，可以有效监测糖尿病小鼠在糖尿病进展过程中肝脏中的Cys浓度并评估糖尿病药物的疗效。我们的工作不仅为 Cys 成像提供了一个出色的工具，而且还引入了设计 NIR-IIb 探头的新概念。

更新日期：2024-09-06

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