Colloidal Hydrogel with Staged Sequestration and Release of Molecules Undergoing Competitive Binding,ACS Nano

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Colloidal Hydrogel with Staged Sequestration and Release of Molecules Undergoing Competitive Binding
ACS Nano ( IF 15.8 ) Pub Date : 2024-09-06 , DOI: 10.1021/acsnano.4c09342
Yuhang Huang ₁ , Nashmia Zia ₂ , Yingshan Ma ₂ , Terek Li ₃ , Gilbert C Walker ₂ , Hani E Naguib _{1,

3,

4,

5} , Eugenia Kumacheva _{1,

2,

5}

Affiliation

Competitive binding of distinct molecules in the hydrogel interior can facilitate dynamic exchange between the hydrogel and the surrounding environment. The ability to control the rates of sequestration and release of these molecules would enhance the hydrogel’s functionality and enable targeting of a specific task. Here, we report the design of a colloidal hydrogel with two distinct pore dimensions to achieve staged, diffusion-controlled scavenging and release dynamics of molecules undergoing competitive binding. The staged scavenging and release strategy was shown for CpG oligodeoxynucleotide (ODN) and human epidermal growth factor (hEGF), two molecules exhibiting different affinities to the quaternary ammonium groups of the hydrogel. Fast ODN scavenging from the ambient environment occurred via diffusion through submicrometer-size hydrogel pores, while delayed hEGF release from the hydrogel was governed by its diffusion through nanometer-size pores. The results of the experiments were in agreement with simulation results. The significance of staged ODN–hEGF exchange was highlighted by the dual anti-inflammation and tissue proliferation hydrogel performance.

中文翻译：

具有阶段性螯合和释放竞争性结合分子的胶体水凝胶

水凝胶内部不同分子的竞争性结合可以促进水凝胶与周围环境之间的动态交换。控制这些分子的封存和释放速率的能力将增强水凝胶的功能并能够靶向特定任务。在这里，我们报告了一种具有两种不同孔径的胶体水凝胶的设计，以实现阶段性、扩散控制的清除和释放竞争性结合分子的动力学。显示了 CpG 寡脱氧核苷酸 (ODN) 和人表皮生长因子 (hEGF) 的分阶段清除和释放策略，这两种分子对水凝胶的季铵基团表现出不同的亲和力。通过亚微米尺寸的水凝胶孔的扩散，从周围环境中快速清除 ODN，而水凝胶中 hEGF 的延迟释放则由其通过纳米尺寸的孔的扩散控制。实验结果与模拟结果一致。水凝胶的双重抗炎和组织增殖性能凸显了分阶段 ODN-hEGF 交换的重要性。

更新日期：2024-09-06

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