Since the discovery of bio-electrosprays, the technology has undergone a rigorous developmental program, which saw the technology exposing to well over 600 cell types ranging from primary, immortalized including stem cells to whole fertilized embryos. Those studies interrogated the post-treated cells in comparison to control cells (cells not exposed to bio-electrosprays) through both well-established clinical read outs (flow cytometry, karyotypic, and gene microarray studies) and biological assays, demonstrating the ability of bio-electrosprays to directly and safely handle the most advanced and complex materials known to humankind, namely, living cells. Since our previous work demonstrated bio-electrospray's ability to jet both human sperm and whole fertilized embryos without damaging them, from a molecular level upward, we wished to investigate if there are any functional effects brought on to the jetted sperm's ability to fertilize oocytes. Therefore, in these investigations, we spearheaded this question by demonstrating for the first time, post-bio-electrosprayed bovine sperm remains motile and viable as assessed, to finally retain their capacity to fertilize oocytes in comparison to controls. These studies pave the way for this platform biotechnology to enter investigations for applications ranging from the development of biological models, sperm analysis/sorting, to their preservation.

中文翻译：

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生物电喷雾牛精子保持活力并使卵母细胞受精


自从生物电喷雾被发现以来，该技术经历了严格的开发计划，该技术暴露于 600 多种细胞类型，从原代细胞、包括干细胞在内的永生细胞到整个受精胚胎。这些研究通过成熟的临床读数（流式细胞术、核型和基因微阵列研究）和生物测定，将处理后的细胞与对照细胞（未暴露于生物电喷雾的细胞）进行比较，证明了生物电喷雾的能力。 -电喷雾直接、安全地处理人类已知的最先进和最复杂的材料，即活细胞。由于我们之前的工作证明了生物电喷雾能够从分子水平向上喷射人类精子和整个受精胚胎而不损坏它们，因此我们希望研究是否会对喷射的精子使卵母细胞受精的能力产生任何功能影响。因此，在这些研究中，我们通过首次证明生物电喷射后的牛精子在评估时仍然具有活动性和活力，与对照组相比，最终保留了其使卵母细胞受精的能力，从而提出了这个问题。这些研究为该平台生物技术进入从生物模型开发、精子分析/分选到保存等应用研究铺平了道路。