Antiviral RNA interference inhibits virus vertical transmission in plants,Cell Host & Microbe

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Antiviral RNA interference inhibits virus vertical transmission in plants
Cell Host & Microbe ( IF 20.6 ) Pub Date : 2024-09-06 , DOI: 10.1016/j.chom.2024.08.009
Si Liu ₁ , Shou-Wei Ding ₁

Affiliation

Known for over a century, seed transmission of plant viruses promotes trans-continental virus dissemination and provides the source of infection to trigger devastating disease epidemics in crops. However, it remains unknown whether there is a genetically defined immune pathway to suppress virus vertical transmission in plants. Here, we demonstrate potent immunosuppression of cucumber mosaic virus (CMV) seed transmission in its natural host Arabidopsis thaliana by antiviral RNA interference (RNAi) pathway. Immunofluorescence microscopy reveals predominant embryo infection at four stages of embryo development. We show that antiviral RNAi confers resistance to seed infection with different genetic requirements and drastically enhanced potency compared with the inhibition of systemic infection of whole plants. Moreover, we detect efficient seed transmission of a mutant CMV lacking its RNAi suppressor gene in mutant plants defective in antiviral RNAi, providing further support for the immunosuppression of seed transmission by antiviral RNAi.

中文翻译：

抗病毒 RNA 干扰抑制病毒在植物中的垂直传播

一个多世纪以来，植物病毒的种子传播促进了病毒的跨大陆传播，并为引发作物毁灭性的疾病流行提供了感染源。然而，是否存在遗传定义的免疫途径来抑制病毒在植物中的垂直传播仍然未知。在这里，我们证明了通过抗病毒 RNA 干扰（RNAi）途径对其天然宿主拟南芥中黄瓜花叶病毒（CMV）种子传播的有效免疫抑制。免疫荧光显微镜检查显示胚胎发育的四个阶段的主要胚胎感染。我们表明，与抑制整株植物的全身感染相比，抗病毒 RNAi 赋予对具有不同遗传要求的种子感染的抵抗力，并且效力大大增强。此外，我们在抗病毒 RNAi 缺陷的突变植物中检测到缺乏 RNAi 抑制基因的突变体 CMV 的高效种子传播，为抗病毒 RNAi 对种子传播的免疫抑制提供了进一步的支持。

更新日期：2024-09-06

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