当前位置: X-MOL 学术Hortic. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
TOR balances plant growth and cold tolerance by orchestrating amino acid-derived metabolism in tomato
Horticulture Research ( IF 7.6 ) Pub Date : 2024-09-05 , DOI: 10.1093/hr/uhae253
Zihao Li 1, 2 , Lin Yang 1, 2 , Yanni Wu 1, 2 , Ran Zhang 1, 2 , Sen Yu 1, 2 , Liwen Fu 1, 2
Affiliation  

The target of rapamycin (TOR) kinase is a central signaling hub that plays a crucial role in precisely orchestrating plant growth, development, and stress responses. This suggests that TOR is intricately involved in maintaining the balance between plant growth and stress responses. Nevertheless, despite the observed effects, the specific mechanisms through which TOR operates in these processes remain obscure. In this study, we investigated how the tomato (Solanum lycopersicum) TOR (SlTOR) affects plant growth and cold responses. We demonstrated that SlTOR inhibition transcriptionally primes cold stress responses, consequently enhancing tomato cold tolerance. A widely targeted metabolomics analysis revealed the disruption of amino acid metabolism homeostasis under cold stress upon SlTOR inhibition, which led to the accumulation of two important cryoprotective metabolites: salicylic acid (SA) and putrescine (Put). Next, we discovered SlPGH1 (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE 1) as a direct substrate of SlTOR. Inhibiting SlTOR led to increased SlCBF1 (C-REPEAT-BINDING FACTOR 1) expression via SlPGH1, potentially triggering the activation of cold-responsive genes and subsequent metabolic alterations. Our study provides a mechanistic framework that elucidates how SlTOR modulates amino acid-related metabolism to enhance tomato cold tolerance, which sheds light on the complex interplay between growth and stress responses orchestrated by TOR.

中文翻译:


TOR 通过协调番茄中氨基酸衍生的代谢来平衡植物生长和耐寒性



雷帕霉素 (TOR) 激酶的靶标是一个中央信号传导枢纽,在精确协调植物生长、发育和胁迫反应中起着至关重要的作用。这表明 TOR 与维持植物生长和胁迫反应之间的平衡有着错综复杂的关系。然而,尽管观察到了效果,但 TOR 在这些过程中运行的具体机制仍然不清楚。在这项研究中,我们调查了番茄 (Solanum lycopersicum) TOR (SlTOR) 如何影响植物生长和寒冷反应。我们证明 SlTOR 抑制转录引发冷应激反应,从而增强番茄的耐寒性。一项广泛靶向的代谢组学分析揭示了 SlTOR 抑制后冷应激下氨基酸代谢稳态的破坏,这导致两种重要的冷冻保护代谢物积累:水杨酸 (SA) 和腐胺 (Put)。接下来,我们发现 SlPGH1 (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE 1) 是 SlTOR 的直接底物。抑制 SlTOR 导致 SlPGH1 的 SlCBF1 (C 重复结合因子 1) 表达增加,可能触发冷反应基因的激活和随后的代谢改变。我们的研究提供了一个机制框架,阐明了 SlTOR 如何调节氨基酸相关代谢以增强番茄的耐寒性,从而阐明了 TOR 编排的生长和胁迫反应之间的复杂相互作用。
更新日期:2024-09-05
down
wechat
bug