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AgI Precipitation Induced Polarity Reversal with Formation of Z-Type Heterojunction for Photoelectrochemical Sensing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-05 , DOI: 10.1021/acs.analchem.4c01577 Chou Wu 1 , Zhipeng Hao 2 , Hanmei Deng 1 , Ying Jiang 1 , Ruo Yuan 1 , Yali Yuan 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-05 , DOI: 10.1021/acs.analchem.4c01577 Chou Wu 1 , Zhipeng Hao 2 , Hanmei Deng 1 , Ying Jiang 1 , Ruo Yuan 1 , Yali Yuan 1
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Regulating photocurrent polarity is highly attractive for fabricating photoelectrochemical (PEC) biosensors with improved sensitivity and accuracy in practical samples. Here, a new approach that adopts the in situ generated AgI precipitate and AgNCs to reversal Bi2WO6 polarity with formation of Z-type heterojunction was proposed for the first time, which coupled with a high-efficient target conversion strategy of exonuclease III (Exo III)-assisted triple recycling amplification for sensing miRNA-21. The target-related DNA nanospheres in situ generated on electrode with loading of plentiful AgI and AgNCs not only endowed the photocurrent of Bi2WO6 switching from the anodic to cathodic one due to the changes in the electron transfer pathway but also formed AgI/AgNCs/Au/Bi2WO6 Z-type heterojunction to improve the photoelectric conversion efficiency for acquiring extremely enhanced PEC signal, thereby significantly avoiding the problem of high background signal derived from traditional unidirectional increasing/decreasing response and false-positive/false-negative. Experimental data showed that the PEC biosensor had a low detection limit down to 0.085 fM, providing a new polarity-reversal mechanism and expected application in diverse fields, including biomedical research and clinical diagnosis.
中文翻译：
AgI 沉淀诱导极性反转并形成 Z 型异质结，用于光电化学传感
调节光电流极性对于制造光电化学（PEC）生物传感器非常有吸引力，可提高实际样品的灵敏度和准确性。在此，首次提出了一种采用原位生成的AgI沉淀和AgNC来反转Bi 2 WO 6极性并形成Z型异质结的新方法，并结合外切核酸酶III的高效靶标转换策略（ Exo III) 辅助三重循环扩增用于感测 miRNA-21。负载大量AgI和AgNCs的电极上原位生成的靶标相关DNA纳米球不仅使Bi 2 WO 6的光电流因电子传递途径的变化而从阳极转换为阴极，而且形成了AgI/AgNCs /Au/Bi 2 WO 6 Z型异质结提高光电转换效率，获得极其增强的PEC信号，从而显着避免传统单向增/减响应带来的高背景信号和假阳性/假阴性的问题。实验数据表明，PEC生物传感器的检测限低至0.085 fM，提供了一种新的极性反转机制，有望在生物医学研究和临床诊断等多个领域得到应用。
更新日期：2024-09-05
中文翻译：
AgI 沉淀诱导极性反转并形成 Z 型异质结，用于光电化学传感
调节光电流极性对于制造光电化学（PEC）生物传感器非常有吸引力，可提高实际样品的灵敏度和准确性。在此，首次提出了一种采用原位生成的AgI沉淀和AgNC来反转Bi 2 WO 6极性并形成Z型异质结的新方法，并结合外切核酸酶III的高效靶标转换策略（ Exo III) 辅助三重循环扩增用于感测 miRNA-21。负载大量AgI和AgNCs的电极上原位生成的靶标相关DNA纳米球不仅使Bi 2 WO 6的光电流因电子传递途径的变化而从阳极转换为阴极，而且形成了AgI/AgNCs /Au/Bi 2 WO 6 Z型异质结提高光电转换效率，获得极其增强的PEC信号，从而显着避免传统单向增/减响应带来的高背景信号和假阳性/假阴性的问题。实验数据表明，PEC生物传感器的检测限低至0.085 fM，提供了一种新的极性反转机制，有望在生物医学研究和临床诊断等多个领域得到应用。
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