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Scavenging of Methylglyoxal by the Total Flavonoids of Apocyni Veneti Folium in Mice
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-04 , DOI: 10.1021/acs.jafc.4c01637 Huixian Zhou 1 , Xinxin Huang 1 , Yun Luo 2 , Ting Tan 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-04 , DOI: 10.1021/acs.jafc.4c01637 Huixian Zhou 1 , Xinxin Huang 1 , Yun Luo 2 , Ting Tan 1
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Scavenging MGO has been considered as an effective strategy for preventing atherosclerosis. A previous study showed that the total flavonoids of Apocyni Veneti Folium (TFAVF) had a significant antiatherosclerotic effect. However, there are no studies that have investigated the MGO scavenging capacities of TFAVF in mice. We found that TFAVF consisted mainly of quercetin glycosides and kaempferol glycosides using ultrahigh performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS). TFAVF was first demonstrated to effectively scavenge MGO in mice based on the formation of mono-MGO-quercetin, mono-MGO-dehydroquercetin, mono-MGO-isorhamnetin, mono-MGO-dehydroisorhamnetin, mono-MGO-kaempferol, and mono-MGO-dehydrokaempferol. In addition, one mono-MGO-quercetin was separated and purified, and its structure was elucidated as 8-MGO-quercetin based on UHPLC-QTOF-MS/MS and NMR data. Quantification studies have demonstrated that kaempferol, dehydrokaempferol, quercetin, dehydroquercetin, isorhamnetin, and dehydroisorhamnetin can dose dependently scavenge MGO in mice. Taken together, these results indicated that TFAVF showed a significant antiatherosclerotic effect, which might be based on MGO detoxification.
中文翻译：
罗布麻叶总黄酮对小鼠体内甲基乙二醛的清除作用
清除MGO已被认为是预防动脉粥样硬化的有效策略。先前的研究表明罗布麻总黄酮（TFAVF）具有显着的抗动脉粥样硬化作用。然而，尚无研究调查 TFAVF 在小鼠体内的 MGO 清除能力。我们利用超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-QTOF-MS/MS）发现TFAVF主要由槲皮素糖苷和山奈酚糖苷组成。基于单-MGO-槲皮素、单-MGO-脱氢槲皮素、单-MGO-异鼠李素、单-MGO-脱氢异鼠李素、单-MGO-山奈酚和单-MGO-脱氢山奈酚。此外，分离纯化了一种单-MGO-槲皮素，并根据UHPLC-QTOF-MS/MS和NMR数据阐明了其结构为8-MGO-槲皮素。定量研究表明，山奈酚、脱氢山奈酚、槲皮素、脱氢槲皮素、异鼠李素和脱氢异鼠李素可以剂量依赖性地清除小鼠中的 MGO。综上所述，这些结果表明 TFAVF 显示出显着的抗动脉粥样硬化作用，这可能是基于 MGO 解毒作用。
更新日期：2024-09-04
中文翻译：
罗布麻叶总黄酮对小鼠体内甲基乙二醛的清除作用
清除MGO已被认为是预防动脉粥样硬化的有效策略。先前的研究表明罗布麻总黄酮（TFAVF）具有显着的抗动脉粥样硬化作用。然而，尚无研究调查 TFAVF 在小鼠体内的 MGO 清除能力。我们利用超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-QTOF-MS/MS）发现TFAVF主要由槲皮素糖苷和山奈酚糖苷组成。基于单-MGO-槲皮素、单-MGO-脱氢槲皮素、单-MGO-异鼠李素、单-MGO-脱氢异鼠李素、单-MGO-山奈酚和单-MGO-脱氢山奈酚。此外，分离纯化了一种单-MGO-槲皮素，并根据UHPLC-QTOF-MS/MS和NMR数据阐明了其结构为8-MGO-槲皮素。定量研究表明，山奈酚、脱氢山奈酚、槲皮素、脱氢槲皮素、异鼠李素和脱氢异鼠李素可以剂量依赖性地清除小鼠中的 MGO。综上所述，这些结果表明 TFAVF 显示出显着的抗动脉粥样硬化作用，这可能是基于 MGO 解毒作用。
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