New point-of-care tests (POCTs), which are especially useful in low-resource settings, are needed to expand screening capacity for diseases that cause significant mortality: tuberculosis, multiple cancers, and emerging infectious diseases. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic (CRISPR-Dx) assays have emerged as powerful and versatile alternatives to traditional nucleic acid tests, revealing a strong potential to meet this need for new POCTs. In this review, we discuss CRISPR-Dx assay techniques that have been or could be applied to develop POCTs, including techniques for sample processing, target amplification, multiplex assay design, and signal readout. This review also describes current and potential applications for POCTs in disease diagnosis and includes future opportunities and challenges for such tests. These tests need to advance beyond initial assay development efforts to broadly meet criteria for use in low-resource settings.

中文翻译：

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CRISPR 在资源匮乏环境中用于伴随诊断


需要新的即时检测 （POCT） 在资源匮乏的环境中特别有用，以扩大对导致大量死亡的疾病的筛查能力：结核病、多种癌症和新发传染病。最近，基于成簇的规则间隔短回文重复序列 （CRISPR） 诊断 （CRISPR-Dx） 检测已成为传统核酸检测的强大且多功能的替代方案，揭示了满足对新 POCT 需求的巨大潜力。在这篇综述中，我们讨论了已经或可能应用于开发 POCT 的 CRISPR-Dx 检测技术，包括样本处理、靶标扩增、多重检测设计和信号读出技术。本综述还描述了 POCTs 在疾病诊断中的当前和潜在应用，并包括此类测试的未来机遇和挑战。这些测试需要超越初始检测开发工作，以广泛满足在资源匮乏环境中使用的标准。