Research on the microbial community and function of the anammox process for environmentally friendly wastewater treatment has achieved certain success, which may mean more universal insights are needed. However, the comprehensive understanding of the anammox process is constrained by the limited taxonomic assignment and functional characterization of anammox microbiota, primarily due to the scarcity of high-quality genomes for most organisms. This study reported a global genome catalog of anammox microbiotas based on numerous metagenomes obtained from both lab- and full-scale systems. A total of 1376 candidate species from 7474 metagenome-assembled genomes were used to construct the genome catalog, providing extensive microbial coverage (averaged of 92.40 %) of anammox microbiota. Moreover, a total of 64 core genera and 44 core species were identified, accounting for approximately 64.25 % and 43.97 %, respectively, of anammox microbiota. The strict core genera encompassed not only functional bacteria (e.g., Brocadia, Desulfobacillus, Zeimonas, and Nitrosomonas) but also two candidate genera (UBA12294 and OLB14) affiliated with the order Anaerolineales. In particular, core denitrifying bacteria with observably taxonomic diversity exhibited diverse functional profiles; for instance, the potential of carbohydrate metabolism in Desulfobacillus and Zeimonas likely improves the mixotrophic lifestyle of anammox microbiota. Besides, a noteworthy association was detected between anammox microbiota and system type. Microbiota in coupling system exhibited complex diversity and interspecies interactions by limiting numerous core denitrifying bacteria. In summary, the constructed catalog substantially expands our understanding of the core community and their functions of anammox microbiota, providing a valuable resource for future studies on anammox systems.

中文翻译：

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包含 1376 个物种水平基因组的综合目录揭示了厌氧氨氧化微生物群落的核心群落和功能多样性


对厌氧氨氧化工艺的微生物群落和功能进行环保废水处理的研究已经取得了一定的成功，这可能意味着需要更普遍的见解。然而，对厌氧氨氧化过程的全面理解受到厌氧氨氧化微生物群有限的分类分配和功能表征的限制，这主要是由于大多数生物体缺乏高质量的基因组。本研究报告了基于从实验室和全尺寸系统获得的大量宏基因组的厌氧氨氧化微生物群的全球基因组目录。总共使用了来自 7474 个宏基因组组装基因组的 1376 个候选物种来构建基因组目录，提供了广泛的微生物覆盖率 （平均 92.40 %）的厌氧氨氧化微生物群。此外，共鉴定出 64 个核心属和 44 个核心种，分别占厌氧氨氧化菌群的 64.25% 和 43.97 %。严格的核心属不仅包括功能性细菌（例如，Brocadia、Desulfobacillus、Zeimonas 和 Nitrosomonas），还包括属于厌氧菌目的两个候选属 （UBA12294 和 OLB14）。特别是，具有可观察到分类多样性的核心反硝化细菌表现出不同的功能特征;例如，脱硫杆菌和 Zeimonas 中碳水化合物代谢的潜力可能会改善厌氧氨氧化微生物群的混合营养生活方式。此外，检测到厌氧氨氧化微生物群与系统类型之间存在显着关联。耦合系统中的微生物群通过限制许多核心反化细菌表现出复杂的多样性和种间相互作用。 总之，构建的目录大大扩展了我们对厌氧氨氧化微生物群核心群落及其功能的理解，为厌氧氨氧化系统的未来研究提供了宝贵的资源。