Rhodosporidium toruloides has emerged as a prominent candidate for producing single-cell oil from cost-effective feedstocks. In this study, the capability of R. toruloides to produce punicic acid (PuA), a representative plant unusual fatty acid, was investigated. The introduction of acyl lipid desaturase and conjugase (PgFADX) allowed R. toruloides to accumulate 3.7 % of total fatty acids as PuA. Delta-12 acyl lipid desaturase (PgFAD2) and diacylglycerol acyltransferase 2 were shown to benefit PuA production. The strain with PgFADX and PgFAD2 coexpression accumulated 12 % of its lipids as PuA from glucose, which translated into a PuA titer of 451.6 mg/L in shake flask condition. Utilizing wood hydrolysate as the feedstock, this strain produced 6.4 % PuA with a titer of 310 mg/L. Taken together, the results demonstrated that R. toruloides could serve as an ideal platform for the production of plant-derived high-value conjugated fatty acid using agricultural and forestry waste as feedstock.

中文翻译：

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工程红冬孢酵母用于从葡萄糖和木材残渣中可持续生产增值石榴酸


圆红冬孢酵母已成为利用具有成本效益的原料生产单细胞油的重要候选者。在本研究中，研究了圆红花（R. toruloides）生产石榴酸（PuA）（一种代表性植物不寻常脂肪酸）的能力。酰基脂质去饱和酶和缀合酶 (PgFADX) 的引入使得 R. toruloides 能够以 PuA 形式积累总脂肪酸的 3.7%。 Delta-12 酰基脂质去饱和酶 (PgFAD2) 和二酰基甘油酰基转移酶 2 有利于 PuA 的产生。 PgFADX 和 PgFAD2 共表达的菌株从葡萄糖中积累了 12% 的脂质以 PuA 形式，在摇瓶条件下转化为 451.6 mg/L 的 PuA 效价。该菌株以木材水解液为原料，产生 6.4% PuA，滴度为 310 mg/L。综上所述，结果表明，R. toruloides 可以作为以农林废弃物为原料生产植物源高价值共轭脂肪酸的理想平台。