Bacterioruberin is widely used in medicine, food, and cosmetics owing to its prominent characteristics of antioxidants and bioactivities. Bioconversion of methane into bacterioruberin is a promising way to address biomanufacturing substrate costs and greenhouse gas emissions but has not been achieved yet. Herein, this study aimed to upcycle methane to bacterioruberin by microbial consortia. The microbial consortia consist of Methylomonas and Methylophilus capable of synthesizing carotenoids from methane was firstly enriched from paddy soil. Through this microbial community, methane was successfully converted into C50 bacterioruberin for the first time. The bioconversion process was then optimized by the response surface methodology. Finally, the methane-derived bacterioruberin reached a record yield of 280.88 ± 2.94 μg/g dry cell weight. This study presents a cost-effective and eco-friendly approach for producing long-chain carotenoids from methane, offering a significant advancement in the direct conversion of greenhouse gases into value-added products.

中文翻译：

---

利用富含土壤的微生物群落从甲烷中可持续生产 C50 类胡萝卜素细菌红素


细菌红素因其突出的抗氧化特性和生物活性而广泛应用于医药、食品和化妆品领域。将甲烷生物转化为细菌红素是解决生物制造底物成本和温室气体排放问题的一种有前途的方法，但尚未实现。在此，本研究旨在通过微生物群落将甲烷升级为细菌红素。由甲基单胞菌和嗜甲基菌组成的能够从甲烷合成类胡萝卜素的微生物群落首先从稻田土壤中富集。通过这个微生物群落，甲烷首次成功转化为C50细菌红素。然后通过响应面方法优化生物转化过程。最终，甲烷衍生的细菌红素产量达到了创纪录的 280.88 ± 2.94 μg/g 细胞干重。这项研究提出了一种利用甲烷生产长链类胡萝卜素的经济高效且环保的方法，在将温室气体直接转化为增值产品方面取得了重大进展。