Lsm14b (LSM family member 14b) is a messenger ribonucleoprotein (mRNP) and a widely present component in eukaryotes. Lsm14b participates in oocyte development by regulating mRNA translation, however, the specific translational regulatory mechanisms remain unclear. Here, we explore the function of Lsm14b during early oocyte development and identify specific translational regulatory mechanisms. We established female-infertile lsm14b mutant zebrafish using CRISPR/Cas9. Histological examination showed that the oocyte development in the mutant zebrafish was arrested at the primary growth (PG) stage. The gene set enrichment analysis (GSEA) analysis of the transcriptome revealed that signaling pathways associated with mRNA translation suppression and mRNA poly(A) tail shortening were significantly downregulation in the mutant. The poly(A) tail length (PAT) assay confirmed the lengthen of mRNA poly(A) tail of the oocyte development-related genes zar1 and figla in the mutant. Further studies have suggested that the loss of Lsm14b triggers the unfolded protein response (UPR), which is related to abnormal translation inhibition. Our results demonstrate that Lsm14b assists in maintaining the translation-inhibited state of mRNA by regulating the length of the mRNA poly(A) tail in zebrafish early oocytes, which ensures that the mRNA synthesized and stored during the growth stage of oocytes, is necessary for the normal growth and development of oocytes.

中文翻译：

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Lsm14b 通过调节 mRNA 聚腺苷酸尾部的聚腺苷酸化来控制斑马鱼卵母细胞的生长


Lsm14b（LSM 家族成员 14b）是一种信使核糖核蛋白 (mRNP)，是真核生物中广泛存在的成分。 Lsm14b通过调节mRNA翻译参与卵母细胞发育，但具体的翻译调节机制仍不清楚。在这里，我们探讨了 Lsm14b 在早期卵母细胞发育过程中的功能，并确定了特定的翻译调节机制。我们使用 CRISPR/Cas9 建立了雌性不育 lsm14b 突变斑马鱼。组织学检查表明，突变斑马鱼的卵母细胞发育在初级生长（PG）阶段被抑制。转录组的基因集富集分析 (GSEA) 分析表明，突变体中与 mRNA 翻译抑制和 mRNA Poly(A) 尾缩短相关的信号通路显着下调。聚腺苷酸尾长度（PAT）测定证实突变体中卵母细胞发育相关基因 zar1 和 figla 的 mRNA 聚腺苷酸尾部延长。进一步的研究表明，Lsm14b 的丢失会触发未折叠蛋白反应（UPR），这与异常翻译抑制有关。我们的结果表明，Lsm14b 通过调节斑马鱼早期卵母细胞中 mRNA 聚腺苷酸尾的长度，协助维持 mRNA 的翻译抑制状态，这确保了卵母细胞生长阶段的 mRNA 合成和储存，对于卵母细胞的正常生长和发育。