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Nitroreductase-Based “Turn-On” Fluorescent Probe for Bacterial Identification with Visible Features
ACS Sensors ( IF 8.2 ) Pub Date : 2024-09-04 , DOI: 10.1021/acssensors.3c02785 Buyue Zhang 1 , Huan Chen 2 , Lei Shi 1 , Ruirui Guo 2 , Yan Wang 2 , Yehuan Zheng 2 , Ruiyang Bai 1 , Yuexing Gao 1 , Bing Liu 2 , Xiufeng Zhang 1
ACS Sensors ( IF 8.2 ) Pub Date : 2024-09-04 , DOI: 10.1021/acssensors.3c02785 Buyue Zhang 1 , Huan Chen 2 , Lei Shi 1 , Ruirui Guo 2 , Yan Wang 2 , Yehuan Zheng 2 , Ruiyang Bai 1 , Yuexing Gao 1 , Bing Liu 2 , Xiufeng Zhang 1
Affiliation
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Among pathogenic bacteria, Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa were the six leading causes for the deaths associated with antibiotic resistance in 2019. Although new treatment options are urgently needed, the precise identification of the bacterial species remains pivotal for an accurate diagnosis and effective treatment. Clinically, mass spectrometry is used to distinguish these bacteria based on their protein mass pattern at the genus and species level. Herein, we report an alternative approach to identify these bacteria using the nitroreductase-based “turn-on” fluorescent probes (ETH1-NO2 and ETH2-NO2), with potential visual indicators for the six individual bacteria species. The limits of detection (LODs) of the probes for NTRs are 0.562 (ETH1-NO2) and 0.153 μg/mL (ETH2-NO2), respectively. They respond effectively to both Gram-positive and Gram-negative bacteria, with the lowest LOD at 1.2 × 106 CFU/mL for E. coli. In particular, different bacteria show noticeable difference in the apparent color of ETH1-NO2 samples, allowing possible identification of these bacteria visually. In addition, ETH1-NO2 also has potential applications in bacterial fluorescence imaging. Thus, our study provides an alternative approach for bacteria identification and new reagents for bacteria imaging.
中文翻译：
基于硝基还原酶的“开启”荧光探针，用于具有可见特征的细菌识别
病原菌中，大肠杆菌、屎肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌和铜绿假单胞菌是2019年抗生素耐药性相关死亡的六大主要原因。细菌种类对于准确诊断和有效治疗仍然至关重要。临床上，质谱法用于根据细菌在属和种水平上的蛋白质质量模式来区分这些细菌。在此，我们报告了一种使用基于硝基还原酶的“开启”荧光探针（ ETH1-NO 2和ETH2-NO 2 ）来识别这些细菌的替代方法，并为六种细菌提供了潜在的视觉指示剂。 NTR 探针的检测限 (LOD) 分别为 0.562 ( ETH1-NO 2 ) 和 0.153 μg/mL ( ETH2-NO 2 )。它们对革兰氏阳性菌和革兰氏阴性菌均有效响应，大肠杆菌的最低 LOD 为 1.2 × 10 6 CFU/mL。特别是，不同的细菌在ETH1-NO 2样品的表观颜色上表现出明显的差异，从而可以通过视觉识别这些细菌。此外， ETH1-NO 2在细菌荧光成像方面也具有潜在的应用。因此，我们的研究提供了细菌鉴定的替代方法和细菌成像的新试剂。
更新日期：2024-09-04
中文翻译：
基于硝基还原酶的“开启”荧光探针，用于具有可见特征的细菌识别
病原菌中，大肠杆菌、屎肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌和铜绿假单胞菌是2019年抗生素耐药性相关死亡的六大主要原因。细菌种类对于准确诊断和有效治疗仍然至关重要。临床上，质谱法用于根据细菌在属和种水平上的蛋白质质量模式来区分这些细菌。在此，我们报告了一种使用基于硝基还原酶的“开启”荧光探针（ ETH1-NO 2和ETH2-NO 2 ）来识别这些细菌的替代方法，并为六种细菌提供了潜在的视觉指示剂。 NTR 探针的检测限 (LOD) 分别为 0.562 ( ETH1-NO 2 ) 和 0.153 μg/mL ( ETH2-NO 2 )。它们对革兰氏阳性菌和革兰氏阴性菌均有效响应，大肠杆菌的最低 LOD 为 1.2 × 10 6 CFU/mL。特别是，不同的细菌在ETH1-NO 2样品的表观颜色上表现出明显的差异，从而可以通过视觉识别这些细菌。此外， ETH1-NO 2在细菌荧光成像方面也具有潜在的应用。因此，我们的研究提供了细菌鉴定的替代方法和细菌成像的新试剂。
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