The best of both worlds: Chemigenetic fluorescent sensors for biological imaging,Cell Chemical Biology

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The best of both worlds: Chemigenetic fluorescent sensors for biological imaging
Cell Chemical Biology ( IF 6.6 ) Pub Date : 2024-09-04 , DOI: 10.1016/j.chembiol.2024.08.002
Kelvin K Tsao ₁ , Shosei Imai ₁ , Michael Chang ₁ , Saaya Hario ₁ , Takuya Terai ₁ , Robert E Campbell ₂

Affiliation

Synthetic-based fluorescent chemosensors and protein-based fluorescent biosensors are two well-established classes of tools for visualizing and monitoring biological processes in living tissues. Chemigenetic sensors, created using a combination of both synthetic parts and protein parts, are an emerging class of tools that aims to combine the strengths, and overcome the drawbacks, of traditional chemosensors and biosensors. This review will survey the landscape of strategies used for fluorescent chemigenetic sensor design. These strategies include: attachment of synthetic elements to proteins using in vitro protein conjugation; attachment of synthetic elements to proteins using autonomous protein labeling; and translational incorporation of unnatural amino acids.

中文翻译：

两全其美：用于生物成像的化学荧光传感器

基于合成的荧光化学传感器和基于蛋白质的荧光生物传感器是两类成熟的工具，用于可视化和监测活组织中的生物过程。化学合成传感器是一类新兴的工具，旨在结合传统化学传感器和生物传感器的优势并克服其缺点。本综述将调查用于荧光化学遗传学传感器设计的策略的前景。这些策略包括：使用体外蛋白质偶联将合成元件附着到蛋白质上;使用自主蛋白质标记将合成元素附着在蛋白质上;和非天然氨基酸的翻译掺入。

更新日期：2024-09-04

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