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Optimization and Validation of a Cheaper, Safer, and More Sustainable Methodology for Aflatoxins Determination in Rich-Lipidic Matrices (Pistachio Nuts) Using Deep Eutectic Solvent Extraction and UHPLC-FLD Analysis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-04 , DOI: 10.1021/acs.jafc.4c05094 Andrea Schincaglia 1, 2 , Luisa Pasti 3 , Alberto Cavazzini 1, 4 , Giorgia Purcaro 2 , Marco Beccaria 1, 5
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-04 , DOI: 10.1021/acs.jafc.4c05094 Andrea Schincaglia 1, 2 , Luisa Pasti 3 , Alberto Cavazzini 1, 4 , Giorgia Purcaro 2 , Marco Beccaria 1, 5
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Aflatoxins pose a major health concern and require strict monitoring in food products. Existing methods rely on hazardous organic solvents for extraction, prompting the development of a greener alternative. This study explores deep eutectic solvents (DESs) for aflatoxin extraction from pistachios, a valuable food product prone to aflatoxin contamination. The proposed method utilizes DES extraction followed by solid-phase extraction cleanup and ultrahigh-performance liquid chromatography coupled with fluorescence detector analysis. Recovery rates ranged from 85.5 to 99.1% for pistachios spiked with 1–8 ng/g aflatoxins, in compliance with EU regulations, with coefficients of variation less than 2.94%. The method demonstrates good sensitivity with limits of detection and quantification in the range of 0.02–0.22 ng/g and 0.05–0.72 ng/g, respectively. Greenness assessment using AGREEPrep and White Analytical Chemistry metrics confirms its environmental sustainability. This approach offers a promising, safer, and more eco-friendly alternative for aflatoxin extraction from complex food matrices like pistachios.
中文翻译：
使用深度共晶溶剂萃取和 UHPLC-FLD 分析，优化和验证更便宜、更安全、更可持续的富脂基质（开心果）中黄曲霉毒素测定方法
黄曲霉毒素造成重大健康问题，需要对食品进行严格监测。现有的方法依赖于有害的有机溶剂进行提取，这促使人们开发出更环保的替代品。本研究探索了从开心果中提取黄曲霉毒素的低共熔溶剂 (DES)，开心果是一种容易受到黄曲霉毒素污染的有价值的食品。该方法采用 DES 萃取，然后进行固相萃取净化和超高效液相色谱结合荧光检测器分析。添加 1-8 ng/g 黄曲霉毒素的开心果的回收率范围为 85.5% 至 99.1%，符合欧盟法规，变异系数小于 2.94%。该方法具有良好的灵敏度，检测限和定量限分别在 0.02–0.22 ng/g 和 0.05–0.72 ng/g 范围内。使用 AGREEPrep 和白色分析化学指标进行的绿色评估证实了其环境可持续性。这种方法为从开心果等复杂食品基质中提取黄曲霉毒素提供了一种有前景、更安全、更环保的替代方案。
更新日期：2024-09-04
中文翻译：
使用深度共晶溶剂萃取和 UHPLC-FLD 分析，优化和验证更便宜、更安全、更可持续的富脂基质（开心果）中黄曲霉毒素测定方法
黄曲霉毒素造成重大健康问题，需要对食品进行严格监测。现有的方法依赖于有害的有机溶剂进行提取，这促使人们开发出更环保的替代品。本研究探索了从开心果中提取黄曲霉毒素的低共熔溶剂 (DES)，开心果是一种容易受到黄曲霉毒素污染的有价值的食品。该方法采用 DES 萃取，然后进行固相萃取净化和超高效液相色谱结合荧光检测器分析。添加 1-8 ng/g 黄曲霉毒素的开心果的回收率范围为 85.5% 至 99.1%，符合欧盟法规，变异系数小于 2.94%。该方法具有良好的灵敏度，检测限和定量限分别在 0.02–0.22 ng/g 和 0.05–0.72 ng/g 范围内。使用 AGREEPrep 和白色分析化学指标进行的绿色评估证实了其环境可持续性。这种方法为从开心果等复杂食品基质中提取黄曲霉毒素提供了一种有前景、更安全、更环保的替代方案。
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