Green Tea Diet Can Effectively Antagonize the Toxicity Induced by Environmental-Related Concentrations of BPA: An Implication from In Vivo and In Silico Studies,Journal of Agricultural and Food Chemistry

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Green Tea Diet Can Effectively Antagonize the Toxicity Induced by Environmental-Related Concentrations of BPA: An Implication from In Vivo and In Silico Studies
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-04 , DOI: 10.1021/acs.jafc.4c05627
Zedong Ouyang ₁ , Weiwei Zhu ₁ , Yuqing Xie ₁ , Wucheng Yang ₁ , Jian Liu ₁ , Qihua Pang ₁ , Ruifang Fan ₁

Affiliation

The neurotoxicity of bisphenol A (BPA) exposure has been confirmed in vitro and in vivo, and inflammatory response is considered the main pathway. Green tea is a healthy life habit as it is rich in various anti-inflammatory components. To confirm that green tea diet is an effective measure to antagonize BPA-induced neurotoxicity, mice were treated with 0.5 and 5000 μg/kg/day of BPA from postnatal days (PNDs) 10–50 and supplemented with green tea on PND 21. From PND 51, behavioral tests were conducted on mice to assess their emotional, cognitive, and spatial learning memory capabilities. The open field test and elevated plus maze test indicated anxiety-like behaviors induced by BPA. Interestingly, green tea diet significantly alleviated BPA-induced anxiety-like behaviors. Meanwhile, the green tea diet effectively reversed BPA-induced microglia activation and morphological changes in the hippocampus of mice. Molecularly, green tea inhibited hippocampal neuroinflammation of mice by reducing BPA-induced expressions of NLRP3, ASC, cleaved-caspase-1, GSDMD-N, IL-6, and IL-1β, as well as significantly reducing the expression of Bak1, Bax, caspase-9, and Cytc c genes (p < 0.05). Molecular docking suggests that various anti-inflammatory components of green tea can competitively bind to the estrogen receptors with BPA. In general, a green tea diet alleviates BPA-induced emotional disorders by inhibiting microglial polarization and hippocampal pyroptosis, indicating its effective antagonistic ability against the neurotoxicity induced by environmental BPA exposure.

中文翻译：

绿茶饮食可以有效对抗环境相关浓度的 BPA 引起的毒性：体内和计算机研究的启示

双酚A（BPA）暴露的神经毒性已在体外和体内得到证实，炎症反应被认为是主要途径。绿茶是一种健康的生活习惯，因为它富含多种抗炎成分。为了证实绿茶饮食是对抗 BPA 诱导的神经毒性的有效措施，从出生后第 10-50 天开始，对小鼠进行 0.5 和 5000 μg/kg/天的 BPA 治疗，并在 PND 21 时补充绿茶。 PND 51，对小鼠进行行为测试，以评估它们的情绪、认知和空间学习记忆能力。旷场测试和高架十字迷宫测试表明 BPA 诱发焦虑样行为。有趣的是，绿茶饮食显着减轻了 BPA 引起的焦虑样行为。同时，绿茶饮食有效逆转了 BPA 诱导的小鼠海马小胶质细胞激活和形态变化。从分子角度来看，绿茶通过降低 BPA 诱导的 NLRP3、ASC、cleaved-caspase-1、GSDMD-N、IL-6 和 IL-1β 的表达，并显着降低 Bak1、Bax 的表达来抑制小鼠海马神经炎症。、caspase-9 和 Cytc c 基因 ( p < 0.05)。分子对接表明，绿茶中的多种抗炎成分可以与 BPA 竞争性地结合雌激素受体。一般来说，绿茶饮食通过抑制小胶质细胞极化和海马焦亡来缓解 BPA 引起的情绪障碍，表明其能够有效对抗环境 BPA 暴露引起的神经毒性。

更新日期：2024-09-04

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